Colomb V, Leturque A, Guihot G, Loizeau M, Lavie S, Colomer S, Ricour C, Girard J
Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, Centre National de la Recherche Scientifique, Meudon-Bellevue, France.
Am J Physiol. 1995 Nov;269(5 Pt 1):E827-33. doi: 10.1152/ajpendo.1995.269.5.E827.
To optimize artificial nutrition (AN) techniques for patients suffering from malnutrition or reduced intestinal absorption, utilization of energy fuels, especially glucose, requires better understanding. Because the liver plays a key role in glucose homeostasis, the aim of this study was to assess the effects of continuous intragastric and intravenous nutrition on insulin secretion and several markers of liver glucose metabolism, especially glucose transporter GLUT-2. Wistar male rats underwent catheterization of either stomach (intragastric) or vena cava (intravenous) and received 24 h/day the same all-in-one formula over 7 to 14 days. The metabolic parameters from intragastrically fed rats did not differ significantly from those from orally fed control rats. Intravenous nutrition induced insulin resistance (marked hyperinsulinemia and/or mild hyperglycemia) and reduced liver GLUT-2 protein and mRNA levels. The decrease in liver GLUT-2 gene expression might be mediated either by an inhibitory role of hyperinsulinemia or by the decrease in gut or portal factors. These results suggest that the route of nutrient delivery influences their utilization by the liver.
为优化针对营养不良或肠道吸收减少患者的人工营养（AN）技术，需要更好地了解能量燃料尤其是葡萄糖的利用情况。由于肝脏在葡萄糖稳态中起关键作用，本研究旨在评估持续胃内和静脉营养对胰岛素分泌及肝脏葡萄糖代谢的几个标志物，尤其是葡萄糖转运蛋白GLUT-2的影响。Wistar雄性大鼠接受胃插管（胃内）或腔静脉插管（静脉内），并在7至14天内每天24小时接受相同的全合一配方。胃内喂养大鼠的代谢参数与经口喂养的对照大鼠无显著差异。静脉营养诱导胰岛素抵抗（显著高胰岛素血症和/或轻度高血糖），并降低肝脏GLUT-2蛋白和mRNA水平。肝脏GLUT-2基因表达的降低可能是由高胰岛素血症的抑制作用或肠道或门静脉因子的减少介导的。这些结果表明营养输送途径会影响肝脏对其的利用。
