Suzawa Y, Hiraoka B Y, Harada M, Deguchi T
Department of Orthodontics, Matsumoto Dental College, Shiojiri-Nagano, Japan.
J Chromatogr B Biomed Appl. 1995 Aug 4;670(1):152-6. doi: 10.1016/0378-4347(95)00162-c.
A simple HPLC procedure for the determination of prolylcarboxypeptidase activity in monkey kidney was established with Cbz-Pro-Ala used as substrate. Decrease of the substrate and increase of the product were stoichiometrically related to each other. Heat treatment at 60 degrees C freed the enzyme preparation of contaminating activities. Data on substrate specificity and influence of inhibitors suggested this method was sensitive for the determination of prolylcarboxypeptidase without the use of a radioactive substrate.
建立了一种简单的高效液相色谱法，以苄氧羰基 - 脯氨酰 - 丙氨酸为底物，用于测定猴肾中的脯氨酰羧肽酶活性。底物的减少和产物的增加在化学计量上相互关联。60℃热处理可去除酶制剂中的污染活性。关于底物特异性和抑制剂影响的数据表明，该方法在不使用放射性底物的情况下对脯氨酰羧肽酶的测定很敏感。
