采用偶氮染料柱前衍生化和胶束液相色谱法测定人尿中的磺胺类药物。

Determination of sulphonamides in human urine by azo dye precolumn derivatization and micellar liquid chromatography.

作者信息

Simó-Alfonso E F, Ramis-Ramos G, García-Alvarez-Coque M C, Esteve-Romero J S

机构信息

Departament de Química Analítica, Facultat de Química, Universitat de València, Spain.

出版信息

J Chromatogr B Biomed Appl. 1995 Aug 4;670(1):183-7. doi: 10.1016/0378-4347(95)00158-f.

DOI:10.1016/0378-4347(95)00158-f

PMID:7493079

Abstract

A high-performance liquid chromatographic method for the determination of sulphonamides in urine is reported. The drugs (sulphadiazine, sulphaguanidine, sulphamethizole, sulphamethoxazole, and sulphathiazole) were diazotized with nitrite and coupled with N-(1-naphthyl)ethylenediamine dihydrochloride in a sodium dodecyl sulphate (SDS) micellar medium. Separation of the sulphonamide azo dyes was performed on a C18 column with a 0.05 M SDS-2.4% pentanol mobile phase, which permitted the direct injection of the urine samples. The limits of detection were in the 0.1-0.3 micrograms/ml range.

摘要

报道了一种测定尿液中磺胺类药物的高效液相色谱法。这些药物（磺胺嘧啶、磺胺脒、磺胺甲噻二唑、磺胺甲恶唑和磺胺噻唑）在亚硝酸钠作用下进行重氮化反应，并在十二烷基硫酸钠（SDS）胶束介质中与盐酸N-（1-萘基）乙二胺偶合。磺胺类偶氮染料在C18柱上，以0.05M SDS - 2.4%戊醇为流动相进行分离，该方法允许直接进样尿液样品。检测限在0.1 - 0.3微克/毫升范围内。