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[冷冻、高温及干燥对斯氏细颈线虫(1896年,雷耶)虫卵及幼虫的影响]

[Effect of freezing, high temperatures and drying on the eggs and larvae of Nematodirus spathiger Railliet, 1896].

作者信息

Zurliĭski P

出版信息

Vet Med Nauki. 1978;15(7):107-16.

PMID:749324
Abstract

A series of laboratory experiments were carried out to test the effect of freezing (--8 to --10 degrees C), high temperatures (15 to 20 degrees C, 26--28 degrees C, 36--37 degrees C, and 58--60 degrees C) on the eggs and larvae of N. spathiger in water, in a dry state, and in the faces. It was found under freezing the eggs retained their viability in water up to 260 days (5.4%), in a dry state up to 330 days (2.8%), and in the feces up to 373 days (5.3%). Embryonated eggs at --8 to --10 degrees C retained their viability up to 440 days. Infective larvae under freezing remained viable in water up to 150 days (4.6%), in a dry state up to 195 days (3.3%), and in the fecal mass up to 230 days (5.2%). Live larvae were detected in the fecal mass on the 450th day (4.6%) when kept at room temperature, on the 395th day (3.5%) at 26--28 degrees C, on the 186th day (5.5%) at 36--37 degrees C, and at the 180th minute (0.8%) at 58--60 degrees C. Frequent wetting and drying of larvae did not produce any unfavourable effect on their viability. A bioassay revealed that infective larvae frozen at --8 to --10 degrees C in the course of 150 days as well as dried at 15--20 degrees C for 166 days remained intact.

摘要

进行了一系列实验室实验,以测试冷冻(-8至-10摄氏度)、高温(15至20摄氏度、26 - 28摄氏度、36 - 37摄氏度和58 - 60摄氏度)对水、干燥状态和粪便中斯氏新线虫卵和幼虫的影响。结果发现,在冷冻条件下,卵在水中可保持活力达260天(5.4%),在干燥状态下可达330天(2.8%),在粪便中可达373天(5.3%)。在-8至-10摄氏度下,已受精的卵可保持活力达440天。冷冻条件下,感染性幼虫在水中可存活达150天(4.6%),在干燥状态下可达195天(3.3%),在粪便团中可达230天(5.2%)。在室温下保存时,粪便团中在第450天检测到活幼虫(4.6%),在26 - 28摄氏度下第395天(3.5%),在36 - 37摄氏度下第186天(5.5%),在58 - 60摄氏度下第180分钟(0.8%)。幼虫频繁的湿润和干燥对其活力未产生任何不利影响。生物测定表明,在-8至-10摄氏度下冷冻150天以及在15 - 20摄氏度下干燥166天的感染性幼虫仍保持完整。

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