Doughty M J, Potvin R, Pritchard N, Fonn D
School of Optometry, University of Waterloo, Ontario, Canada.
Doc Ophthalmol. 1995;89(4):355-71. doi: 10.1007/BF01203711.
Fluorescein dye is commonly used to highlight the tarsal conjunctiva in man to facilitate diagnosis of papillary conjunctivitis. A quantitative analysis of the fluorescein-highlighted features could be useful in both assignment of severity grades as well as for objective comparisons but no data is available on the distributions of sizes (areas) of these features. An exploratory, range-finding study was undertaken to provide data on feature areas that might be encountered. After application of fluorescein, 35 mm photographs were taken at a fixed distance with zone 1/zone 2 (Allansmith) of the tarsal plate aligned perpendicular to the camera. The photographs were projected, an overlay of 150 to 180 contiguous features outlined by fluorescein made and planimetry used to assess the areas and area distributions of the features on tarsal plates clinically graded with stages 1 to 4 papillary hypertrophy. For zone 1 of the tarsal plate in asymptomatic subjects, a fluorescein solution highlights the limits of features that enclose an area averaging 30,000 microns2 (range 8000 to 120,000; median area of close to 35,000 microns2). The areas of these features were generally normally distributed and there was little variation in feature areas across the zone. At different stages of clinical papillary conjunctivitis, the areas of the negative staining patterns are larger. Averages of 60,000 to 90,000 microns2, median values of close to 70,000 microns2 and individual feature areas up to 350,000 microns2 were seen in mildly symptomatic patients. These distributions were generally heterogeneous and showed a skewed distribution. Areas averaging up to 0.755 mm2 (range 0.6 to 3.2 mm2) were measured in moderate to severe papillary hypertrophy.
荧光素染料常用于在人体中突出睑结膜，以促进乳头性结膜炎的诊断。对荧光素突出显示的特征进行定量分析，对于严重程度分级以及客观比较都可能有用，但目前尚无关于这些特征大小（面积）分布的数据。因此进行了一项探索性的范围查找研究，以提供可能遇到的特征面积数据。应用荧光素后，在固定距离拍摄35毫米照片，使睑板的1区/2区（阿兰史密斯区）与相机垂直对齐。将照片投影，绘制出由荧光素勾勒出的150至180个连续特征的叠加图，并使用面积测量法评估临床上分级为1至4期乳头肥大的睑板上特征的面积和面积分布。对于无症状受试者睑板的1区，荧光素溶液突出显示了包围平均面积为30,000平方微米（范围为8000至120,000；中位数面积接近35,000平方微米）区域的特征边界。这些特征的面积通常呈正态分布，并且该区域内特征面积变化很小。在临床乳头性结膜炎的不同阶段，阴性染色模式的面积更大。在轻度症状患者中，平均面积为60,000至90,000平方微米，中位数接近70,000平方微米，个别特征面积可达350,000平方微米。这些分布通常是不均匀的，呈偏态分布。在中度至重度乳头肥大中，测量到的平均面积高达0.755平方毫米（范围为0.6至3.2平方毫米）。
