László F, Whittle B J
Wellcome Foundation Ltd., Beckenham, Kent, UK.
Eur J Pharmacol. 1995 Apr 24;277(2-3):R1-3. doi: 10.1016/0014-2999(95)00158-h.
Administration of the nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (5 mg/kg s.c.) provoked acute microvascular injury (assessed by the leakage of radiolabelled human serum albumin) in the rat colon within 1 h, when administered concurrently with endotoxin (Escherichia coli lipopolysaccharide, 3 mg/kg i.v.). Pretreatment with the selective inhibitor of 5-lipoxygenase, BW A137C (N-[4-benzyloxybenzyl] acetohydroxamic acid; 1-20 mg/kg s.c., 15 min before endotoxin) attenuated such damage in a dose-dependent manner. These findings suggest a balance between protective constitutive nitric oxide and the detrimental actions of 5-lipoxygenase products in the maintenance of vascular integrity in the early stage of sepsis.
一氧化氮合酶抑制剂NG-硝基-L-精氨酸甲酯（5毫克/千克，皮下注射）与内毒素（大肠杆菌脂多糖，3毫克/千克，静脉注射）同时给药时，在1小时内可引发大鼠结肠急性微血管损伤（通过放射性标记的人血清白蛋白渗漏评估）。用5-脂氧合酶的选择性抑制剂BW A137C（N-[4-苄氧基苄基]乙酰氧肟酸；1-20毫克/千克，皮下注射，在内毒素注射前15分钟）预处理可剂量依赖性地减轻这种损伤。这些发现表明，在脓毒症早期维持血管完整性方面，保护性组成型一氧化氮与5-脂氧合酶产物的有害作用之间存在平衡。
