Riparbelli M G, Callaini G, Tripodi S A, Cintorino M, Tosi P, Dallai R
Department of Evolutionary Biology, University of Siena, Italy.
Exp Cell Res. 1995 Dec;221(2):363-9. doi: 10.1006/excr.1995.1386.
Incubation with the Bcl-2 antibody before fixation of tissues allowed good localization of the antigenic determinant. We showed that the Bcl-2 gene product in centroblastic-centrocytic lymphoma is mainly localized on the outer mitochondrial membrane and, to a lesser degree, on the nuclear envelope. No significant staining was found in other cytoplasmic domains. Careful examination also revealed that gold particles did not recognize an integral membrane epitope, but an antigenic determinant localized at a short distance from the cytoplasmic side of the membrane itself. This observation suggests that, by interacting with other cytoplasmic proteins, Bcl-2 plays some role in the cytoplasmic machinery involved in the regulation of programmed cell death.
在组织固定前用Bcl-2抗体孵育可使抗原决定簇得到良好定位。我们发现，中心母细胞-中心细胞淋巴瘤中的Bcl-2基因产物主要定位于线粒体外膜，其次定位于核膜。在其他细胞质区域未发现明显染色。仔细检查还发现，金颗粒识别的不是完整膜表位，而是位于距膜细胞质侧短距离处的抗原决定簇。这一观察结果表明，通过与其他细胞质蛋白相互作用，Bcl-2在参与程序性细胞死亡调节的细胞质机制中发挥了一定作用。
