Barthold S W, Levy S A, Fikrig E, Bockenstedt L K, Smith A L
Section of Comparative Medicine, School of Medicine, Yale University, New Haven, CT 06520-8016, USA.
J Am Vet Med Assoc. 1995 Dec 1;207(11):1435-40.
To characterize the serologic responses of dogs naturally exposed to or vaccinated against Borrelia burgdorferi and to assess responses at intervals after antibiotic treatment.
Prospective, controlled clinical trial.
19 dogs of various breeds and ages with narrowly defined clinical criteria of limb/joint borreliosis and 10 control dogs of equivalent age were used to determine serologic responses following natural exposure to the organism. Eight seronegative dogs were used to determine serologic responses following vaccination.
Serologic responses to B burgdorferi and recombinant outer surface protein (Osp)A, flagellin, and P39 were assessed by means of ELISA and western immunoblot. Passive protective activity was assessed by use of a mouse protection assay.
Naturally exposed dogs were seropositive, but had variable ELISA titers and immunoblot profiles. Immunoblot analysis did reveal consistent reactions to flagellin, P39, and a 22 kd protein, but not to OspA. Antibody responses did not change appreciably up to 13 weeks after antibiotic treatment. Vaccinated dogs had strong reactions to OspA and OspB, but not to P39.
Dogs with clinical borreliosis are seropositive and remain seropositive after antibiotic treatment, emphasizing that serologic testing is not a useful means of measuring clinical response. Serologic responses of infected dogs can be discriminated from those of vaccinated dogs by means of immunoblot analysis, and recombinant P39 is a potentially useful antigen for that purpose.
描述自然暴露于伯氏疏螺旋体或接种过该菌疫苗的犬的血清学反应，并评估抗生素治疗后不同时间间隔的反应。
前瞻性对照临床试验。
19只不同品种和年龄、符合肢体/关节莱姆病狭义临床标准的犬，以及10只年龄相当的对照犬，用于确定自然暴露于该病原体后的血清学反应。8只血清阴性犬用于确定接种疫苗后的血清学反应。
通过酶联免疫吸附测定（ELISA）和western免疫印迹法评估对伯氏疏螺旋体、重组外膜蛋白（Osp）A、鞭毛蛋白和P39的血清学反应。通过小鼠保护试验评估被动保护活性。
自然暴露的犬血清呈阳性，但ELISA滴度和免疫印迹图谱各不相同。免疫印迹分析确实显示对鞭毛蛋白、P39和一种22kd蛋白有一致反应，但对OspA无反应。抗生素治疗后长达13周，抗体反应没有明显变化。接种疫苗的犬对OspA和OspB有强烈反应，但对P39无反应。
患有临床莱姆病的犬血清呈阳性，抗生素治疗后仍保持血清阳性，这强调血清学检测不是衡量临床反应的有用方法。通过免疫印迹分析可以区分感染犬和接种疫苗犬的血清学反应，重组P39可能是用于此目的的有用抗原。
