Pender N, Heaney T G
Department of Clinical Dental Sciences, School of Dentistry, University of Liverpool, U.K.
J Periodontal Res. 1995 Sep;30(5):312-8. doi: 10.1111/j.1600-0765.1995.tb01281.x.
The aim of this investigation was to determine whether gingival fibroblast progenitor cells undergo division and whether there is evidence of migration of their progeny from the paravascular sites within the interdental gingival septum (IGS). 30 male hooded Lister rats aged 6 weeks, were killed in groups of ten, 3, 75 and 171 h after a single injection of tritiated thymidine. Autoradiographs were examined of transverse Historesin sections of the papilla between second and third mandibular molars in 29 specimens, taken from equidistant intervals between the col and alveolar bone crest. Median grain counts in the whole papilla were greatest within 10 microns of blood vessels at 3 h (p < 0.02) and decreased throughout the papilla with time (p < 0.0001). The median grain counts at level 5, nearest the periodontal ligament, were greatest at all times (p < 0.01). The distance of labelled nuclei from blood vessel walls increased with time, most notably at level 5 (p < 0.001). The mean Clustering Index increased (p < 0.01) from 4.6% at 3 h to 12.3% and 11.6% at 75 h and 171 h respectively. At 75 h, the median grain count of clustered nuclei was smaller (p < 0.01) than that of non-clustered nuclei. These data are consistent with the occurrence of clonal division and migration within the IGS and the presence of a slowly cycling cell population near to the periodontal ligament.
本研究的目的是确定牙龈成纤维细胞祖细胞是否会发生分裂，以及是否有证据表明其子代细胞从牙间牙龈隔（IGS）内的血管旁位点迁移。30只6周龄的雄性带帽利斯特大鼠，在单次注射氚标记胸腺嘧啶核苷后，分别于3、75和171小时，每组10只处死。对29个标本中下颌第二和第三磨牙之间乳头的横向Historesin切片进行放射自显影片检查，这些切片取自龈谷和牙槽嵴之间等距间隔处。在3小时时，整个乳头内距血管10微米范围内的中位颗粒计数最高（p<0.02），且随时间在整个乳头内减少（p<0.0001）。在最靠近牙周膜的5级水平处，中位颗粒计数在所有时间都是最高的（p<0.01）。标记细胞核与血管壁的距离随时间增加，在5级水平最为明显（p<0.001）。平均聚集指数从3小时时的4.6%增加（p<0.01），分别在75小时和171小时时增加到12.3%和11.6%。在75小时时，聚集细胞核的中位颗粒计数低于非聚集细胞核（p<0.01）。这些数据与IGS内发生克隆分裂和迁移以及牙周膜附近存在缓慢循环细胞群一致。
