Laurie D J, Putzke J, Zieglgänsberger W, Seeburg P H, Tölle T R
Center for Molecular Biology (ZMBH), University of Heidelberg, Germany.
Brain Res Mol Brain Res. 1995 Aug;32(1):94-108. doi: 10.1016/0169-328x(95)00067-3.
Regional variation in the alternative splice forms of the NMDAR1 subunit mRNA was investigated by in situ hybridization in the adult rat brain, using radiolabelled splice-specific oligonucleotide probes. Each splice variant was detected in an individual distribution. The NMDAR1-a and NMDAR1-2 forms were widely and abundantly distributed throughout the brain, except for the inferior colliculus. The NMDAR1-b and NMDAR1-4 variants were located in similar patterns in fewer areas (e.g. parietal cortex, hippocampus CA3, thalamus, inferior colliculus, cerebellar granule cells). In contrast, the NMDAR1-1 forms were distributed in a pattern approximately complementary in the forebrain to that of NMDAR1-4 (weakly expressed in thalamus and inferior colliculus). The NMDAR1-3 variants were not abundant in any structure. Considerable overlap of the in situ hybridization images was noted, so all eight splice combinations are possible in heterogenous distributions. Correlation of the distribution of NMDAR1 mRNA splice forms with functional analyses of heteromeric recombinant receptors will be necessary to ascertain if alternative splicing of the NMDAR1 subunit can account for some of the known heterogeneity of endogenous NMDA receptors.
利用放射性标记的剪接特异性寡核苷酸探针，通过原位杂交技术在成年大鼠脑中研究了NMDAR1亚基mRNA可变剪接形式的区域差异。每种剪接变体都有其独特的分布。NMDAR1-α和NMDAR1-2形式在整个大脑中广泛且大量分布，但下丘除外。NMDAR1-β和NMDAR1-4变体在较少的区域（如顶叶皮质、海马CA3、丘脑、下丘、小脑颗粒细胞）呈现相似的分布模式。相比之下，NMDAR1-1形式在前脑的分布模式与NMDAR1-4大致互补（在丘脑和下丘中弱表达）。NMDAR1-3变体在任何结构中都不丰富。原位杂交图像存在相当程度的重叠，因此在异质分布中所有八种剪接组合都是可能的。要确定NMDAR1亚基的可变剪接是否能解释内源性NMDA受体一些已知的异质性，有必要将NMDAR1 mRNA剪接形式的分布与异聚体重组受体的功能分析进行关联。
