Chenthamarakshan V, Reddy M V, Harinath B C
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Wardha, India.
Parasite Immunol. 1995 Jun;17(6):277-85. doi: 10.1111/j.1365-3024.1995.tb00893.x.
A 120 kDa antigen containing SDS-PAGE fraction BmA-2 isolated from Brugia malayi adult parasite was highly reactive with normal sera from filarial endemic area. BmA-2 was analysed for its prophylactic potential in in vitro and in vivo. Sera collected from BmA-2 immunized jirds induced a significant level (80 to 90%) of protection against infective larvae and microfilariae in in vitro ADCC assay as well as in in situ micropore chamber implantation studies. Mastomys natalensis immunized with BmA-2 showed a significant level of protective response against circulating microfilariae by clearing 90% of them from circulation by fifth day after challenge infection. Immunization of jirds with BmA-2 resulted in an enhanced level of antibody response against BmA-2 and 88% reduction in the development of the parasites to the adult stage. Passive transfer of immunesera from jirds immunized with BmA-2 to naive jirds resulted in 71% reduction in adult worm recovery as observed 90 days after challenge infection with B. malayi. On the other hand the passive transfer of non-adherent spleen cells from immune jirds did not show any significant effect on the development of parasite. Administration of jirds anti BmA-2 serum to microfilaraemic jirds showed a temporary decrease in microfilarial count which was increased to pretherapeutic level within 100 days and there was no effect on the adult worms. This implies that the immune protective effect of BmA-2 is mainly antibody dependent and active immunization with BmA-2 is effective against filarial infection.
从马来布鲁线虫成虫中分离出的含有SDS-PAGE组分BmA-2的120 kDa抗原与丝虫病流行地区的正常血清具有高度反应性。对BmA-2的体外和体内预防潜力进行了分析。在体外ADCC试验以及原位微孔室植入研究中，从用BmA-2免疫的沙鼠收集的血清诱导了对感染性幼虫和微丝蚴的显著水平（80%至90%）的保护作用。用BmA-2免疫的南非多乳鼠对循环微丝蚴表现出显著水平的保护反应，在攻击感染后第5天，循环中的微丝蚴清除率达90%。用BmA-2免疫沙鼠导致针对BmA-2的抗体反应水平增强，寄生虫发育至成虫阶段的比例降低了88%。将用BmA-2免疫的沙鼠的免疫血清被动转移至未免疫的沙鼠，在感染马来布鲁线虫攻击感染90天后观察到成虫回收率降低了71%。另一方面，将免疫沙鼠的非贴壁脾细胞进行被动转移，对寄生虫的发育未显示出任何显著影响。给微丝蚴血症沙鼠注射抗BmA-2血清，微丝蚴计数暂时下降，100天内又回升至治疗前水平，对成虫无影响。这表明BmA-2的免疫保护作用主要依赖抗体，用BmA-2进行主动免疫对丝虫感染有效。
