Kohler H P, Müller M, Bombeli T, Straub P W, Haeberli A
Department of Medicine, University Hospital of Bern, Switzerland.
Thromb Haemost. 1995 Apr;73(4):719-24.
Human umbilical vein endothelial cells (HUVEC) were cultivated on globular microcarriers in order to improve the endothelial cell surface to blood-volume ratio over the conventional flat bed cultures. HUVEC-beads were tested for their modulation of blood coagulation using a combination of two steps: HUVEC-beads were added into the syringe used for the venipuncture, in order to achieve immediate contact between cells and blood, and no anticoagulant was used during the incubation time of HUVEC-beads with whole blood. The coagulation initiation produced by venipuncture was almost completely suppressed as judged by thrombin measurements over a period of 60 min. The activated partial thromboplastin time showed a prolongation by a factor > 3. Direct measurements of activated protein C (APC) were negative. Moreover, inhibition of APC-generation with a monoclonal anti-human protein C antibody did not affect the anticoagulant properties of endothelial cells. Therefore the anticoagulant properties exerted by HUVEC-beads are not dependent on APC.
人脐静脉内皮细胞（HUVEC）在球状微载体上培养，以提高内皮细胞表面积与血容量的比率，优于传统的平板培养。使用两个步骤的组合测试HUVEC微珠对血液凝固的调节作用：将HUVEC微珠添加到用于静脉穿刺的注射器中，以使细胞与血液立即接触，并且在HUVEC微珠与全血孵育期间不使用抗凝剂。通过在60分钟内测量凝血酶判断，静脉穿刺产生的凝血起始几乎被完全抑制。活化部分凝血活酶时间延长了>3倍。活化蛋白C（APC）的直接测量结果为阴性。此外，用单克隆抗人蛋白C抗体抑制APC生成并不影响内皮细胞的抗凝特性。因此，HUVEC微珠发挥的抗凝特性不依赖于APC。
