Pandey P C, Weetall H H
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Appl Biochem Biotechnol. 1995 Nov;55(2):87-94. doi: 10.1007/BF02783551.
A flow-injection analysis (FIA) system coupled with an evanescent wave (EW) biosensor employing total internal reflection of fluorescence radiation (TIRF) for the detection of polyaromatic hydrocarbon that intercalates into DNA is reported. A highly fluorescent intercalator, "ethidium bromide," has been used as the reference compound for detection. The EW biosensor was developed according to the procedure described earlier (1,2). Data on the analysis of Naphthalene, 3-methylcholanthrene, 7,12-dimethylbenz(a)anthracene, 1,2-benzanthracene, and some standard reference materials supplied by the National Institute of Standards and Technology are reported. The relative ability of the polyaromatic hydrocarbon to displace ethidium bromide, based on the relative binding ratio, is found to be on the order of 7,12-dimethylbenz[a]anthracene > 3-methylcholanthrene > 1,2-benzanthracene > napthalene.
报道了一种流动注射分析（FIA）系统，该系统与一种倏逝波（EW）生物传感器相结合，利用荧光辐射的全内反射（TIRF）来检测嵌入DNA的多环芳烃。一种高荧光嵌入剂“溴化乙锭”已被用作检测的参考化合物。EW生物传感器是按照先前描述的程序开发的（1,2）。报告了萘、3-甲基胆蒽、7,12-二甲基苯并[a]蒽、1,2-苯并蒽以及美国国家标准与技术研究院提供的一些标准参考物质的分析数据。基于相对结合率，发现多环芳烃取代溴化乙锭的相对能力顺序为7,12-二甲基苯并[a]蒽＞3-甲基胆蒽＞1,2-苯并蒽＞萘。
