de Waal Malefyt R, Abrams J S, Zurawski S M, Lecron J C, Mohan-Peterson S, Sanjanwala B, Bennett B, Silver J, de Vries J E, Yssel H
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304-1104, USA.
Int Immunol. 1995 Sep;7(9):1405-16. doi: 10.1093/intimm/7.9.1405.
In the present study, the requirements and characteristics for the production of IL-13 by human T cells, T cell clones and B cells were determined and compared with those of IL-4. IL-13 was produced by human CD4+ and CD8+ T lymphocyte subsets isolated from peripheral blood mononuclear cells and by CD4+ and CD8+ T cell clones. CD4+ T cell clones belonging to Th0, Th1-like and Th2-like subsets produced IL-13 following antigen-specific or polyclonal activation. In addition, EBV-transformed B cell lines expressed IL-13 mRNA and produced small amounts of IL-13 protein. Expression of IL-13 mRNA and production of IL-13 protein by peripheral blood T cells and T cell clones was induced rapidly and was relatively long lasting, whereas IL-4 production by these cells was transient. In addition, IL-13 mRNA expression was induced by modes of activation that failed to induce IL-4 mRNA expression. IL-13 shares many biological activities with IL-4 which is compatible with the notion that the IL-13 and IL-4 receptors share a common component required for signal transduction. However, IL-13 lacks the T cell-activating properties of IL-4. Here we have shown that this is related to the fact that T cells fail to bind radiolabeled IL-13 and do not express the IL-13-specific receptor component. Taken together, these results indicate that the differences in expression and biological activities of IL-4 and IL-13 on T cells may have consequences for the relative roles of these cytokines in the immune response.
在本研究中，确定了人T细胞、T细胞克隆和B细胞产生白细胞介素-13（IL-13）的条件和特性，并与白细胞介素-4（IL-4）的条件和特性进行了比较。从外周血单个核细胞中分离出的人CD4⁺和CD8⁺T淋巴细胞亚群以及CD4⁺和CD8⁺T细胞克隆可产生IL-13。属于Th0、Th1样和Th2样亚群的CD4⁺T细胞克隆在抗原特异性或多克隆激活后产生IL-13。此外，EB病毒转化的B细胞系表达IL-13信使核糖核酸（mRNA）并产生少量IL-13蛋白。外周血T细胞和T细胞克隆中IL-13 mRNA的表达和IL-13蛋白的产生诱导迅速且持续时间相对较长，而这些细胞产生IL-4是短暂的。此外，未能诱导IL-4 mRNA表达的激活方式可诱导IL-13 mRNA表达。IL-13与IL-4具有许多生物学活性，这与IL-13和IL-4受体共享信号转导所需的共同成分这一观点相符。然而，IL-13缺乏IL-4的T细胞激活特性。我们在此表明，这与T细胞不能结合放射性标记的IL-13且不表达IL-13特异性受体成分这一事实有关。综上所述，这些结果表明IL-4和IL-13在T细胞上表达和生物学活性的差异可能会影响这些细胞因子在免疫反应中的相对作用。
