Morré D J, Fleurimont J, Sweeting M
Department of Medicinal Chemistry and Pharmacognosy, Purdue University, West Lafayette, IN 47907, USA.
Biochim Biophys Acta. 1995 Nov 22;1240(1):5-9. doi: 10.1016/0005-2736(95)00177-5.
Elongation of soybean hypocotyl sections induced by the auxin herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D), was blocked by micromolar concentrations of the sulfonylurea herbicide chlorsulfuron (2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-trizin-2- yl)amino]carbonyl]benzenesulfonamide). The inhibition at equimolar concentrations of 2,4-D and chlorsulfuron was overcome by a 10- or 100-fold excess of 2,4-D. Stimulation by 2,4-D of an 2,4-D-responsive NADH-oxidase activity of the soybean plasma membrane also was blocked by the presence of concentrations of chlorsulfuron equimolar to the 2,4-D. Chlorsulfuron alone was largely without effect on either cell elongation or the auxin-stimulated NADH-oxidase activity over a range of chlorsulfuron concentrations. The results show a clear correlation between inhibition of auxin-stimulated cell elongation and the inhibition of an auxin-stimulated component of a plasma membrane NADH-oxidase activity.
生长素除草剂2,4-二氯苯氧乙酸（2,4-D）诱导的大豆下胚轴切段伸长，被微摩尔浓度的磺酰脲类除草剂氯磺隆（2-氯-N-[[(4-甲氧基-6-甲基-1,3,5-三嗪-2-基)氨基]羰基]苯磺酰胺）所阻断。等摩尔浓度的2,4-D和氯磺隆的抑制作用可被过量10倍或100倍的2,4-D克服。氯磺隆等摩尔浓度存在时，2,4-D对大豆质膜的2,4-D响应型NADH氧化酶活性的刺激作用也被阻断。在一系列氯磺隆浓度范围内，单独的氯磺隆对细胞伸长或生长素刺激的NADH氧化酶活性基本没有影响。结果表明，生长素刺激的细胞伸长抑制与质膜NADH氧化酶活性的生长素刺激成分的抑制之间存在明显的相关性。
