Scotet E, Houssaint E
INSERM U 211, Institut de Biologie, Nantes, France.
Biochim Biophys Acta. 1995 Nov 7;1264(2):238-42. doi: 10.1016/0167-4781(95)00156-b.
An essential feature of fibroblast growth factor receptors (FGFR) is the existence of multiple possibilities for alternative splicing. One of these concerns sequences of the mRNA coding for the C-terminal half of Ig domain 3 which corresponds to a part of the ligand-binding site. Two alternative exons, IIIb and IIIc, encode the C-terminal half of Ig domain 3. We show here that the alternative splicing choice between IIIb and IIIc exons of the FGFR-3 is not strictly tissue-specific: epithelial cells show exclusively IIIb transcripts while fibroblastic cells show a mixture of IIIb and IIIc transcripts. This is in contrast with the strictly exclusive alternative choice between IIIb or IIIc exons of the FGFR-2 gene: epithelial cells make only the IIIb choice while fibroblastic cells make only the IIIc choice.
成纤维细胞生长因子受体（FGFR）的一个基本特征是存在多种可变剪接的可能性。其中之一涉及编码免疫球蛋白结构域3（Ig结构域3）C端一半的mRNA序列，该序列对应于配体结合位点的一部分。两个可变外显子IIIb和IIIc编码Ig结构域3的C端一半。我们在此表明，FGFR-3的IIIb和IIIc外显子之间的可变剪接选择并非严格的组织特异性：上皮细胞仅显示IIIb转录本，而成纤维细胞则显示IIIb和IIIc转录本的混合物。这与FGFR-2基因的IIIb或IIIc外显子之间严格排他性的可变选择形成对比：上皮细胞仅进行IIIb选择，而成纤维细胞仅进行IIIc选择。
