Vigorito E, Robles A, Balter H, Nappa A, Goñi F
Cátedra de Immunologia, Facultad de Química, Universidad de la República Oriental del Uruguay, Montevideo.
Appl Radiat Isot. 1995 Oct;46(10):975-9. doi: 10.1016/0969-8043(95)00208-u.
In order to study the interaction between an IgM cold agglutinin and the erythrocyte I antigen, the former antibody was labelled with 125I using the Chloramine-T, IODOGEN and Bolton-Hunter methods. High incorporation and adequate stability of the labelled IgM were obtained with all procedures. However, suitable biological activity was maintained only with the Bolton-Hunter method. Further studies suggest that tyrosine iodination affects antigen recognition by this IgM, whereas iodination of amino groups does not. The reagent thus prepared allowed the determination of the number of I sites per erythrocyte as well as the antibody affinity constant.
为了研究IgM冷凝集素与红细胞I抗原之间的相互作用，使用氯胺-T法、碘代甘氨酸法和博尔顿-亨特法用125I标记前者抗体。所有方法均获得了高掺入率和标记IgM的足够稳定性。然而，只有博尔顿-亨特法能保持合适的生物学活性。进一步的研究表明，酪氨酸碘化会影响该IgM对抗原的识别，而氨基碘化则不会。如此制备的试剂可用于测定每个红细胞上I位点的数量以及抗体亲和常数。
