de Crécy-Lagard V, Binet M, Danchin A
Unité de Régulation de l'Expression génétique, Institut Pasteur, Paris, France.
Microbiology (Reading). 1995 Sep;141 ( Pt 9):2253-60. doi: 10.1099/13500872-141-9-2253.
In the plant pathogen Xanthomonas campestris pv. campestris, fructose is transported by a specific phosphotransferase system (PTS). This PTS involves a multiphosphoryl transfer protein (MTP) encoded by the fruB gene, which was cloned and sequenced. fruB is part of a transcriptional unit together with the fruK gene, coding for 1-phosphofructokinase, which is located upstream from the fruA gene, coding for the fructose-specific permease (EIIB'BCFru). The amino acid sequence of the X. campestris MTP deduced from the fruB sequence shared 46% identical residues with an MTP identified in Rhodobacter capsulatus. The X. campestris MTP (837 amino acid residues) consists of three moieties: a fructose-specific enzyme-IIA-like N-terminal moiety (residues 1-148), followed by an HPr-like moiety (161-251) and an enzyme-I-like C-terminal moiety (274-837). The three domains are separated by two flexible hinge regions rich in proline and alanine residues. The construction of a fruB mutant confirmed the role of the MTP in fructose transport and phosphorylation in X. campestris.
在植物病原菌野油菜黄单胞菌野油菜致病变种中，果糖通过一种特定的磷酸转移酶系统（PTS）进行转运。该PTS涉及由fruB基因编码的多磷酸转移蛋白（MTP），此基因已被克隆并测序。fruB与fruK基因共同构成一个转录单元，fruK基因编码1-磷酸果糖激酶，它位于fruA基因的上游，fruA基因编码果糖特异性通透酶（EIIB'BCFru）。从fruB序列推导的野油菜黄单胞菌MTP的氨基酸序列与在荚膜红细菌中鉴定出的一种MTP有46%的相同残基。野油菜黄单胞菌MTP（837个氨基酸残基）由三个部分组成：一个果糖特异性的酶-IIA样N端部分（残基1-148），接着是一个HPr样部分（161-251）和一个酶-I样C端部分（274-837）。这三个结构域由两个富含脯氨酸和丙氨酸残基的柔性铰链区隔开。fruB突变体的构建证实了MTP在野油菜黄单胞菌果糖转运和磷酸化中的作用。
