Alexeyev M F, Shokolenko I N, Croughan T P
Louisiana State University Agricultural Center, Rice Research Station, Crowley 70527-1429, USA.
Can J Microbiol. 1995 Nov;41(11):1053-5. doi: 10.1139/m95-147.
Five mini-Tn5 derivatives encoding resistance to Km, Cm, Gm, Tc, and Sm, coupled with the polylinker of the pBluescriptII plasmid, were constructed. These derivatives are carried by an ampicillin-resistant plasmid that has a conditional origin of replication from plasmid R6K and origin of conjugal transfer from the broad host range plasmid RP4. The new vectors are smaller than those previously described and possess numerous unique restriction sites inside the minitransposons for gene cloning in addition to SfiI and NotI sites found in their predecessors.
构建了五个编码对卡那霉素（Km）、氯霉素（Cm）、庆大霉素（Gm）、四环素（Tc）和链霉素（Sm）耐药性的mini-Tn5衍生物，并与pBluescriptII质粒的多克隆位点相连。这些衍生物由一个抗氨苄青霉素的质粒携带，该质粒具有来自质粒R6K的条件复制起点和来自广宿主范围质粒RP4的接合转移起点。新载体比先前描述的载体更小，并且除了其前身中发现的SfiI和NotI位点外，mini转座子内部还拥有许多用于基因克隆的独特限制酶切位点。
