Angelova M B, Genova L K, Slokoska L S, Pashova S B
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Can J Microbiol. 1995 Nov;41(11):978-83. doi: 10.1139/m95-136.
The effect of glucose on superoxide dismutase (SOD) activity produced in Humicola lutea 110 was determined. The relatively high glucose concentration in the medium did not repress SOD levels in the cells. Glycerol, a nonfermentable carbon source, caused a slight stimulation of SOD synthesis. Furthermore, the specific rates of enzyme production in the medium with different glucose concentration showed an insignificant difference. Cyclic AMP had no effect on SOD levels. The shift in metabolism as glucose was depleted resulted in an increase in the rate of synthesis of both isocitric dehydrogenase and SOD. Pentachlorophenol and paraquat, which cause the production of superoxide radicals, caused an increase in SOD activity. These results led us to conclude that it is superoxide ion rather than glucose that is controlling SOD levels.
测定了葡萄糖对黄孢原毛平革菌110产生的超氧化物歧化酶（SOD）活性的影响。培养基中相对较高的葡萄糖浓度并未抑制细胞中的SOD水平。甘油，一种不可发酵的碳源，对SOD的合成有轻微的刺激作用。此外，不同葡萄糖浓度培养基中酶的比生产速率显示出无显著差异。环磷酸腺苷对SOD水平没有影响。随着葡萄糖耗尽，代谢转变导致异柠檬酸脱氢酶和SOD的合成速率增加。导致超氧自由基产生的五氯苯酚和百草枯会使SOD活性增加。这些结果使我们得出结论，控制SOD水平的是超氧离子而非葡萄糖。
