George J, Kuttan R
Amala Cancer Research Centre, Amala Nagar, India.
Cancer Lett. 1995 Nov 6;97(2):213-6. doi: 10.1016/0304-3835(95)03968-3.
Tetramethyl thiuram disulphide (Thiram, TMTD), a mutagen, is analysed for its clastogenic property by using micronuclei induction studies in the bone marrow of erythrocytes of male Swiss albino mice. Three concentrations of TMTD, i.e. 100, 150 and 200 mg/kg body wt did not significantly increase the micronuclei in polychromatic erythrocytes or the ratio of poly-to normochromatic erythrocytes. The tumour initiating (10 mg and 1 mg each in 0.2 ml DMSO) and promoting (1 mg in 0.2 ml DMSO) potency of TMTD were tested in female Swiss mice by two stage skin carcinogenic model. There was no significant increase in tumours in the TMTD treated groups, either as initiator or promoter. The results indicated nonclastogenicity and non-carcinogenicity of TMTD under these experimental conditions.
四甲基二硫化秋兰姆（福美双，TMTD）是一种诱变剂，通过在雄性瑞士白化小鼠红细胞骨髓中进行微核诱导研究来分析其致断裂特性。三种浓度的TMTD，即100、150和200毫克/千克体重，并未显著增加多染性红细胞中的微核或多染性红细胞与正染性红细胞的比例。通过两阶段皮肤致癌模型在雌性瑞士小鼠中测试了TMTD的肿瘤起始能力（分别在0.2毫升二甲基亚砜中各含10毫克和1毫克）和促进能力（在0.2毫升二甲基亚砜中含1毫克）。无论是作为起始剂还是促进剂，TMTD处理组的肿瘤均未显著增加。结果表明在这些实验条件下TMTD无致断裂性和无致癌性。
