George J, Kuttan R
Amala Cancer Research Centre, Amala Nagar, Thrissur, India.
Mutat Res. 1996 Oct 1;370(3-4):191-4. doi: 10.1016/s0165-1218(96)00072-9.
N-(1,3-Dimethylbutyl)-N-phenylparaphenylenediamine (DMPPD) is a derivative of phenylenediamine (PPD) which is widely used in the rubber industry as an antioxidant. DMPPD which is a strong allergen, is least studied for its clastogenic potency. This study evaluated the genotoxic property of DMPPD in Swiss albino mice bone marrow cells by using chromosomal aberration and micronuclei assay. Three concentrations of DMPPD, i.e., 100, 150 and 200 mg/kg body wt. did not significantly increase the micronuclei in polychromatic erythrocytes or the ratio of poly to normochromatic erythrocytes. Chromosome aberration studies using 100 mg and 200 mg/kg body wt. showed that DMPPD is a non-inducer of chromosome aberrations. The results indicated non-clastogenicity of DMPPD in mice marrow cells up to a concentration of 200 mg/kg body wt. under our experimental conditions.
N-（1,3-二甲基丁基）-N-苯基对苯二胺（DMPPD）是对苯二胺（PPD）的衍生物，在橡胶工业中广泛用作抗氧化剂。DMPPD是一种强过敏原，但其致断裂潜能的研究最少。本研究通过染色体畸变和微核试验评估了DMPPD对瑞士白化小鼠骨髓细胞的遗传毒性。三种浓度的DMPPD，即100、150和200mg/kg体重，并未显著增加多色红细胞中的微核或多色与正色红细胞的比例。使用100mg/kg和200mg/kg体重进行的染色体畸变研究表明，DMPPD不是染色体畸变的诱导剂。结果表明，在我们的实验条件下，浓度高达200mg/kg体重时，DMPPD在小鼠骨髓细胞中无致断裂性。
