来自流感嗜血杆菌galEgalK菌株的细胞包膜脂寡糖糖类部分的结构研究。

Structural studies of the saccharide part of the cell envelope lipooligosaccharide from Haemophilus influenzae strain galEgalK.

作者信息

Schweda E K, Jansson P E, Moxon E R, Lindberg A A

机构信息

Karolinska Institute, Clinical Research Centre, Huddinge Hospital, Sweden.

出版信息

Carbohydr Res. 1995 Aug 11;272(2):213-24. doi: 10.1016/0008-6215(95)00018-o.

DOI:10.1016/0008-6215(95)00018-o

PMID:7497478

Abstract

The structure of the saccharide part of the lipooligosaccharide from Haemophilus influenzae strain galEgalK has been investigated. On treatment of the lipooligosaccharide with acid under mild conditions, followed by reduction with sodium borohydride and gel permeation chromatography, a main fraction was obtained which was studied by methylation analysis, NMR spectroscopy, and FABMS. The material was heterogeneous and contained two major compounds, A and B, and one minor, C. [formula: see text] In the structure, PEA is phosphoethanolamine, and L-D-Hep is L-glycero-D-manno-heptose. Kdo exists in reduced anhydro forms. The carbohydrate backbone is the same as that proposed for the saccharide part of the major component from H. influenzae type b strain A2 [N.J. Phillips, M. A. Apicella, J. M. Griffiss, and B. W. Gibson, Biochemistry, 32 (1993) 2003-2012].

摘要

已对来自流感嗜血杆菌galEgalK菌株的脂寡糖的糖部分结构进行了研究。在温和条件下用酸处理脂寡糖，随后用硼氢化钠还原并进行凝胶渗透色谱法，得到了一个主要级分，通过甲基化分析、核磁共振光谱法和快原子轰击质谱法对其进行了研究。该物质具有异质性，包含两种主要化合物A和B以及一种次要化合物C。[化学式：见正文]在该结构中，PEA为磷酸乙醇胺，L-D-Hep为L-甘油-D-甘露庚糖。Kdo以还原脱水形式存在。碳水化合物主链与b型流感嗜血杆菌A2菌株主要成分的糖部分所提出的结构相同[N.J.菲利普斯、M.A.阿皮塞拉、J.M.格里菲斯和B.W.吉布森，《生物化学》，32(1993)2003-2012]。