Risberg A, Schweda E K, Jansson P E
Clinical Research Centre, Karolinska Institutet, Huddinge Hospital, NOVUM, Sweden.
Eur J Biochem. 1997 Feb 1;243(3):701-7. doi: 10.1111/j.1432-1033.1997.00701.x.
The structure of the oligosaccharide part of the Haemophilus influenzae RM.118-28 lipopolysaccharide (LPS) has been investigated. The oligosaccharide was obtained from the LPS by mild acid hydrolysis followed by gel-permeation chromatography, and was studied by methylation analysis, NMR spectroscopy and mass spectrometry. The structure of the major compound, which is a hexasaccharide, is proposed as follows. [formula: see text] In the structure, Kdo is 3-deoxy-D-manno-octulosonic acid, PEtn is phosphoethanolamine, PCho is phosphocholine and L,D-Hep is L-glycero-D-manno-heptose. Electrospray-ionization mass spectrometry on O-deacylated LPS obtained after treatment with anhydrous hydrazine gave evidence for the presence of two minor compounds, which show additional substitution of the main structure with phosphate and PEtn, respectively. These substitutions have not been localized.
对流感嗜血杆菌RM.118 - 28脂多糖（LPS）寡糖部分的结构进行了研究。通过温和酸水解，然后进行凝胶渗透色谱法从LPS中获得寡糖，并通过甲基化分析、核磁共振光谱和质谱对其进行研究。主要化合物（一种六糖）的结构如下所示。[化学式：见原文] 在该结构中，Kdo为3 - 脱氧 - D - 甘露糖辛酸，PEtn为磷酸乙醇胺，PCho为磷酸胆碱，L，D - Hep为L - 甘油 - D - 甘露庚糖。在用无水肼处理后得到的O - 脱酰基LPS上进行电喷雾电离质谱分析，结果表明存在两种次要化合物，它们分别显示出主结构上额外的磷酸化和磷酸乙醇胺取代。这些取代位置尚未确定。
