Immunosuppressive effect of concanavalin-A. II. In vivo suppression of cytotoxic lymphocytes generation in alloimmunized mice.

An examination was carried out of the in vivo effect of Concanavalin-A (Con-A) on the induction of secondary cytotoxic response (CL) to allogeneic spleen cells in mice. CL responses were suppressed by one i.v. injection of 200 micrograms of Con-A one day before first or second immunization or two i.v. injections of Con-A, 200 micrograms each, given simultaneously with immunization. Single dose of 200 microgram of Con-A given simultaneously inhibited anamnestic CL response only partially, to the range observed in the primary response.