Gravel P, Sanchez J C, Walzer C, Golaz O, Hochstrasser D F, Balant L P, Hughes G J, Garcia-Sevilla J, Guimon J
Clinical Research Unit, Psychiatric University Institutions of Geneva, Switzerland.
Electrophoresis. 1995 Jul;16(7):1152-9. doi: 10.1002/elps.11501601191.
Two-dimensional (2-D) maps of cytosol and enriched-membrane platelet proteins has allowed the identification of more than 25 spots by three different methods: matching of the platelet gels with other 2-D reference maps, immunoblotting with chemiluminescence detection, and N-terminal sequencing. Different G protein (guanosine triphosphate-binding protein) subunits, cytoskeletal proteins, and proteins common to the human liver, red blood cells and plasma were identified. The two platelet protein maps presented here contribute to the project of identification of human cell and body fluid proteins. They may serve as working tools since platelets are popular models for the study of central nervous system neurotransmitter systems and stimulus-response coupling mechanisms.
通过三种不同方法，即血小板凝胶与其他二维参考图谱的匹配、化学发光检测免疫印迹以及N端测序，已得到了胞质溶胶和富含膜的血小板蛋白的二维图谱，从而识别出了25个以上的斑点。已鉴定出不同的G蛋白（鸟苷三磷酸结合蛋白）亚基、细胞骨架蛋白以及人肝脏、红细胞和血浆共有的蛋白。这里展示的这两张血小板蛋白图谱有助于人类细胞和体液蛋白的鉴定项目。由于血小板是研究中枢神经系统神经递质系统和刺激-反应偶联机制的常用模型，它们可用作研究工具。
