Miller I, Gutleb A C, Gemeiner M
Institut für Medizinische Chemie, Veterinärmedizinische Universität Wien, Osterreich.
Electrophoresis. 1995 Jul;16(7):1193-8. doi: 10.1002/elps.11501601199.
Studies on serum/blood samples from European otters (Lutra lutra), a species under protection in most countries, were undertaken. Building of a two-dimensional electrophoresis (2-DE) protein identification map was started, identifying serum proteins by pattern comparison with cat or human serum protein maps and by immunoblotting using cross-reacting antibodies against the respective human serum proteins. The capture of endangered species is very restricted, thus making fresh samples almost unavailable; most investigations therefore had to be performed on samples collected from animals found dead. Patterns obtained from specimens of this source were compared to the established pattern of serum proteins and of blood or liver cells. Some of the major differences observed can be explained by contamination with cellular proteins and/or the onset of lytic processes generating breakdown products, as shown by preliminary in vitro experiments. Better knowledge of "normal" serum protein patterns and interfering products will facilitate interpretation of data collected from samples with a less defined history.
对欧洲水獭（Lutra lutra）的血清/血液样本进行了研究，欧洲水獭在大多数国家都受到保护。开始构建二维电泳（2-DE）蛋白质鉴定图谱，通过与猫或人类血清蛋白质图谱进行模式比较，以及使用针对相应人类血清蛋白质的交叉反应抗体进行免疫印迹来鉴定血清蛋白质。濒危物种的捕获受到严格限制，因此几乎无法获得新鲜样本；因此，大多数研究不得不对从死亡动物身上采集的样本进行。将从该来源标本获得的模式与已建立的血清蛋白质以及血液或肝细胞模式进行比较。初步体外实验表明，观察到的一些主要差异可以用细胞蛋白质污染和/或产生分解产物的溶解过程的开始来解释。更好地了解“正常”血清蛋白质模式和干扰产物将有助于解释从历史不太明确的样本中收集的数据。
