Emaduddin M, Liu G J, Takeuchi H
Department of Physiology, Gifu University School of Medicine, Japan.
Eur J Pharmacol. 1995 Sep 5;283(1-3):113-24. doi: 10.1016/0014-2999(95)00301-z.
The pharmacological features of dopamine receptors in identifiable giant neurone types of a snail (Achatina fulica Férussac) were studied. Under voltage clamp, two neurone types, LVMN (left ventral multiple spike neurone) and d-RPeAN (dorsal-right pedal anterior neurone), produced an inward current (Iin) in response to dopamine, (-)-noradrenaline and epinine, whereas v-LCDN (ventral-left cerebral distinct neurone) produced an outward current (Iout) in response to dopamine and epinine. Mammalian dopamine receptor agonists, fenoldopam (dopamine D1-like receptor agonist), (+/-)-SKF 38393 (1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine-7,8- diol) (D1-like), apomorphine (D2-like), (-)-quinpirole (D3 and D4) and methylergometrine showed slight or no effect. (+/-)-SKF 83566 ((+/-)-7-bromo-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benza zepine) (dopamine D1-like receptor antagonist) and (+)-UH 232 (cis-(+)-5-methoxy-1-methyl-2-(di-n-propylamino)tetralin) (D3 and D2) non-competitively inhibited the Iin of LVMN and d-RPeAN, but (+/-)-sulpiride (D2-like) was without effect. In contrast, (+/-)-sulpiride competitively inhibited Iout of v-LCDN, (+)-UH 232 non-competitively inhibited Iout of v-LCDN but (+/-)-SKF 83566 was without effect. H-7 (1-(5-isoquinolinesulfonyl)-2-methylpiperazine) (non-selective protein kinase inhibitor) inhibited Iin of LVMN and d-RPeAN, but did not affect Iout of v-LCDN. Dopamine-induced Iin was Na(+)-dependent; Iout was K(+)-dependent. Ouabain did not affect these currents. We propose that the pharmacological features of Achatina neuronal dopamine receptors are not fully comparable to those of mammals, although intracellular signal transduction systems linked with dopamine receptors may similarly exist in different animal species.
