Nishikawa M, Omay S B, Nakai K, Kihira H, Kobayashi T, Tamura S, Shiku H
2nd Department of Internal Medicine, Mie University School of Medicine, Japan.
FEBS Lett. 1995 Nov 20;375(3):299-303. doi: 10.1016/0014-5793(95)01209-w.
Treatment with 20 nM 1 alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3) caused a progressive increase in the activity of Mg(2+)-dependent protein serine/threonine phosphatase type 2C (PP2C) in subcellular fractions of HL-60 cells, whereas PP2C activity was relatively constant throughout all-trans retinoic acid-induced (1 microM) granulocytic differentiation. The increase in PP2C activity appeared to parallel the 1,25(OH)2D3-induced phenotypic and functional changes in HL-60 cells. Immunoblot and Northern blot analysis indicated that the increase in PP2C activity corresponded to the increased expression of PP2C protein, which was preceded by an increase in the level of mRNA for PP2C beta. No mRNA for PP2C alpha was detected in resting or 1,25(OH)2D3-stimulated HL-60 cells. These results suggest that the increased expression of PP2C is related with the 1,25(OH)2D3-induced monocytic differentiation of HL-60 cells.
用20 nM 1α,25 - 二羟基维生素D3（1,25(OH)2D3）处理导致HL - 60细胞亚细胞组分中Mg(2+)依赖性蛋白丝氨酸/苏氨酸磷酸酶2C（PP2C）的活性逐渐增加，而在全反式维甲酸诱导（1 μM）的粒细胞分化过程中，PP2C活性相对恒定。PP2C活性的增加似乎与1,25(OH)2D3诱导的HL - 60细胞的表型和功能变化平行。免疫印迹和Northern印迹分析表明，PP2C活性的增加对应于PP2C蛋白表达的增加，这之前PP2Cβ的mRNA水平有所增加。在静止或1,25(OH)2D3刺激的HL - 60细胞中未检测到PP2Cα的mRNA。这些结果表明，PP2C表达的增加与1,25(OH)2D3诱导的HL - 60细胞单核细胞分化有关。
