Fritsch M, Geilen C C, Heidrich C, Reutter W
Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Berlin-Dahlem, Germany.
FEBS Lett. 1995 Dec 4;376(3):159-63. doi: 10.1016/0014-5793(95)01265-8.
A cell culture model was developed to investigate the involvement of gangliosides in cell-matrix adhesion. Two cell lines with different adhesive properties derived from solid Morris hepatoma 7777 were established. Cultured in horse serum-containing medium, the adhesive cell line (MH 7777A) adheres and spreads on uncoated culture dishes, whereas the revertant cell line (MH 7777A > N) does not adhere and grows in suspension. The adhesiveness of both cell lines is dependent on the coating protein used (none, bovine serum albumin, fibronectin or collagen I) and the horse serum concentration in the culture medium. Both cell lines, although of the same origin, differed in their ganglioside composition. The most abundant ganglioside of both MH 7777A and MH 7777A > N cell lines was fucosyl-GM1, 0.78 and 0.72 microgram per mg cellular protein, respectively. The GM3 and GD1a content of MH 7777A > N cells was significantly higher than that of MH 7777A cells. Furthermore, a matrix-dependency of the ganglioside pattern of both cell lines was demonstrated.
建立了一种细胞培养模型，以研究神经节苷脂在细胞与基质黏附中的作用。从实体瘤莫里斯肝癌7777中获得了两种具有不同黏附特性的细胞系。在含马血清的培养基中培养时，黏附性细胞系（MH 7777A）能在未包被的培养皿上黏附并铺展，而回复细胞系（MH 7777A > N）不黏附并悬浮生长。两种细胞系的黏附性取决于所用的包被蛋白（无、牛血清白蛋白、纤连蛋白或I型胶原）以及培养基中的马血清浓度。两种细胞系虽然来源相同，但神经节苷脂组成不同。MH 7777A和MH 7777A > N细胞系中最丰富的神经节苷脂是岩藻糖基-GM1，分别为每毫克细胞蛋白0.78和0.72微克。MH 7777A > N细胞的GM3和GD1a含量显著高于MH 7777A细胞。此外，还证明了两种细胞系神经节苷脂模式的基质依赖性。
