佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）诱导的大鼠腹腔中性粒细胞氧化爆发可被0.1 mT（60 Hz）的磁场增强。
The phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field.
作者信息
Roy S, Noda Y, Eckert V, Traber M G, Mori A, Liburdy R, Packer L
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
出版信息
FEBS Lett. 1995 Dec 4;376(3):164-6. doi: 10.1016/0014-5793(95)01266-x.
Magnetic fields (MF) may affect biological systems by increasing free radical concentrations. To test this, we have investigated whether low frequency (60 Hz) low intensity (0.1 mT) MF can modulate the phorbol 12-myristate 13- acetate (PMA) induced respiratory burst in primed rat peritoneal neutrophils, followed in real time using the dye 2',7'-dichlorofluorescin (DCFH), which reacts with free radical-derived oxidants such as H2O2 (which is formed from the dismutation of superoxide) to become 2',7'-dichlorofluorecein (DCF), a highly fluorescent compound. In the presence of the MF, a 12.4% increase in the fluorescence signal was observed in PMA-stimulated neutrophils (n = 5, P < 0.02, 18 pairs of measurements). We believe this represents the first experimental observation of MF influencing events involving free radical species generated during signal transduction in living cells.
磁场（MF）可能通过增加自由基浓度来影响生物系统。为了验证这一点，我们研究了低频（60Hz）低强度（0.1mT）的MF是否能够调节佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）诱导的致敏大鼠腹腔中性粒细胞的呼吸爆发，实时跟踪使用染料2'，7'-二氯荧光素（DCFH），其与自由基衍生的氧化剂如H2O2（由超氧化物歧化形成）反应生成2'，7'-二氯荧光素（DCF），一种高荧光化合物。在MF存在的情况下，在PMA刺激的中性粒细胞中观察到荧光信号增加了12.4%（n = 5，P < 0.02，18对测量值）。我们认为这代表了MF影响活细胞信号转导过程中涉及自由基产生的事件的首次实验观察。
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