Safronova V G, Gapeev A B, Alovskaia A A, Gabdulkhakova A G, Chemeris N K, Fesenko E E
Institute of Cell Biophysics, Russian Acad. Sci., Pushchino, Moscow Region.
Biofizika. 1997 Nov-Dec;42(6):1267-73.
The effect of extremely high frequency electromagnetic field (mm-waves) on respiratory burst of neutrophils was studied. The peritoneal evoked neutrophils of the mice (NMRI line) were used. The production of reactive oxygen species was estimated by luminol-dependent chemiluminescence technique. Cells were irradiated by the mm-waves of 41. 95 GHz in the far field zone of the channel radiator during 20 min. Absorbed energy flux density was 150 microW/cm2. The irradiation was carried out at different concentrations of calcium ionophore A23187 and then neutrophils were stimulated by phorbol 12-myristate 13-acetate (PMA) 1 microM, activator of PKC. At irradiation of neutrophils the synergistic action of A23187 and PMA was not changed at low concentration of ionophore 10 nM-0.5 microM and was suppressed at high concentrations 0.5-10 microM. The largest inhibition of about 60% was obtained at the concentration of A23187 20 microM. The effect of mm-waves was not found under exposure in Ca(2+)-free medium for all used A23187 concentrations. We suggest that the mm-wave effect on the production of reactive oxygen species by neutrophils is determined by the influx of extracellular Ca2+ and PKC.
研究了极高频电磁场（毫米波）对中性粒细胞呼吸爆发的影响。使用了小鼠（NMRI品系）腹膜诱发的中性粒细胞。通过鲁米诺依赖性化学发光技术评估活性氧的产生。细胞在通道辐射器的远场区域接受41.95GHz毫米波照射20分钟。吸收的能量通量密度为150微瓦/平方厘米。在不同浓度的钙离子载体A23187存在下进行照射，然后用1微摩尔/升佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）刺激中性粒细胞，PMA是蛋白激酶C的激活剂。在照射中性粒细胞时，在低浓度离子载体10纳摩尔/升至0.5微摩尔/升时，A23187和PMA的协同作用不变，而在高浓度0.5微摩尔/升至10微摩尔/升时受到抑制。在A23187浓度为20微摩尔/升时，获得了约60%的最大抑制率。对于所有使用的A23187浓度，在无钙培养基中暴露时未发现毫米波的影响。我们认为，毫米波对中性粒细胞活性氧产生的影响是由细胞外Ca2+和蛋白激酶C的内流决定的。
