Uusitalo M, Kivelä T
Department of Opthalmology, Helsinki University Central Hospital, Finland.
Invest Ophthalmol Vis Sci. 1995 Dec;36(13):2584-91.
To analyze the cytoskeletal development in neuroectodermally derived epithelial and muscle cells of the human eye during the second and third trimesters of pregnancy.
Nine formalin-fixed, paraffin-embedded fetal autopsy eyes from the 13th to 40th week of gestation were studied with eight monoclonal antibodies (mAbs) to intermediate filaments and alpha-smooth muscle actin (alpha SMA) by the avidin-biotinylated-peroxidase complex method.
The epithelium of the iris reacted with mAbs Vim 3B4 and V9 to vimentin in all eyes. Reactivity with mAb CAM 5.2 to cytokeratin (CK) 8 and mAb CY-90 and KS-B17.2 to CK 18 disappeared from the iris epithelium by the 28th gestational week. mAb 1A4 to alpha SMA labeled its anterior layer from the 28th week on. mAbs DE-U-10 and D33 to desmin labeled dilator fibers by the 37th week, and focal reactivity for CK 8 and 18 concurrently appeared. The developing iris sphincter reacted for alpha SMA in all eyes. It was labeled increasingly for desmin from the 18th week on, whereas initial reactivity for vimentin gradually disappeared after the 22nd week. mAbs to vimentin, CK 8, and CK 18 labeled the ciliary epithelium and the retinal pigment epithelium in all eyes, except for lack of CK 8 and 18 in the nonpigmented ciliary epithelium at the 13th week. The ciliary muscle reacted uniformly for vimentin and alpha SMA and from the 16th week on for desmin.
The results highlight the individuality of cytoskeletal profiles in the neuroectodermal epithelial and muscle cells of the eye and clarify the formation of the peculiar cytoskeleton typical of the adult human eye. They also offer a framework for detecting cytoskeletal changes in developmental anomalies of the eye after the first trimester of pregnancy.
分析妊娠中期和晚期人眼神经外胚层来源的上皮细胞和肌肉细胞中的细胞骨架发育情况。
采用抗生物素蛋白-生物素化过氧化物酶复合物法，用8种针对中间丝和α-平滑肌肌动蛋白（α-SMA）的单克隆抗体（mAb）对9例妊娠13至40周福尔马林固定、石蜡包埋的胎儿尸检眼进行研究。
所有眼中，虹膜上皮细胞与抗波形蛋白的单克隆抗体Vim 3B4和V9发生反应。到妊娠第28周时，虹膜上皮细胞与抗细胞角蛋白（CK）8的单克隆抗体CAM 5.2、抗CK 18的单克隆抗体CY-90和KS-B17.2的反应消失。抗α-SMA的单克隆抗体1A4从第28周开始标记虹膜前层。抗结蛋白的单克隆抗体DE-U-10和D33到第37周时标记瞳孔开大肌纤维，同时出现对CK 8和18的局灶性反应。所有眼中发育中的虹膜括约肌对α-SMA有反应。从第18周开始，其结蛋白标记逐渐增加，而波形蛋白的初始反应在第22周后逐渐消失。除第13周无色素睫状体上皮缺乏CK 8和18外，抗波形蛋白、CK 8和CK 18的单克隆抗体标记所有眼中的睫状体上皮和视网膜色素上皮。睫状肌对波形蛋白和α-SMA呈均匀反应，从第16周开始对结蛋白有反应。
结果突出了眼神经外胚层上皮细胞和肌肉细胞中细胞骨架分布的个体差异，阐明了成人眼典型特殊细胞骨架的形成。它们还为检测妊娠前三个月后眼部发育异常中的细胞骨架变化提供了一个框架。
