Ghazvini S, Kroll S, Char D H, Frigillana H
Department of Ophthalmology, University of California, San Francisco 94143, USA.
Invest Ophthalmol Vis Sci. 1995 Dec;36(13):2762-7.
Recent production of a monoclonal antibody, PC10, against proliferating cell nuclear antigen (PCNA) makes it possible to evaluate cell cycling in formalin-fixed tissues. In this study, the authors quantitatively evaluated the relationship between PCNA expression and two other measures of cell cycling, bromodeoxyuridine labeling index (BrdU LI) and mitotic index (MI), in archival uveal melanomas. The authors also examined the relative prognostic importance of each measure.
Serial sections from 35 formalin-fixed, paraffin-embedded uveal melanomas were immunostained with PC10 and BrdU antibody using a standard avidin-biotin-peroxidase method. A quantitative scoring system was used to evaluate the fraction of cells that were positive for PCNA, BrdU, and mitotic figures in the regions of high cycling. The LIs of the different markers were compared, and their prognostic importance was evaluated.
The median PCNA LI was 3.05% compared to the median BrdU LI of 0.94% and the median MI of 0.034%. The PCNA LI was more variable in replicate sections than either the MI or the BrdU LI. The correlation between PCNA LI and BrdU LI was 0.58, between PCNA LI and MI it was 0.46, and between BrdU LI and MI it was 0.81. The relative risk of tumor-related mortality per doubling of BrdU LI was 2.35, and of MI it was 2.34. Although these were significant, PCNA LI of 1.08 was not.
Proliferating cell nuclear antigen immunostaining did not demonstrate a strong relationship with either BrdU LI or MI. Unlike MI and BrdU LI, PCNA LI was not correlated with tumor-related mortality. Caution is warranted in the interpretation of PCNA immunostaining in uveal melanomas.
近期生产出一种针对增殖细胞核抗原（PCNA）的单克隆抗体PC10，这使得评估福尔马林固定组织中的细胞周期成为可能。在本研究中，作者定量评估了存档葡萄膜黑色素瘤中PCNA表达与另外两种细胞周期指标——溴脱氧尿苷标记指数（BrdU LI）和有丝分裂指数（MI）之间的关系。作者还研究了每种指标相对的预后重要性。
采用标准抗生物素蛋白-生物素-过氧化物酶方法，用PC10和BrdU抗体对35例福尔马林固定、石蜡包埋的葡萄膜黑色素瘤的连续切片进行免疫染色。使用定量评分系统评估高细胞周期区域中PCNA、BrdU和有丝分裂图阳性细胞的比例。比较不同标志物的LI，并评估其预后重要性。
PCNA LI的中位数为3.05%，而BrdU LI的中位数为0.94%，MI的中位数为0.034%。PCNA LI在重复切片中的变异性比MI或BrdU LI更大。PCNA LI与BrdU LI之间的相关性为0.58，PCNA LI与MI之间为0.46，BrdU LI与MI之间为0.81。BrdU LI每增加一倍，肿瘤相关死亡的相对风险为2.35，MI为2.34。虽然这些具有显著性，但PCNA LI为1.08时则不然。
增殖细胞核抗原免疫染色与BrdU LI或MI均未显示出强相关性。与MI和BrdU LI不同，PCNA LI与肿瘤相关死亡率无关。在解释葡萄膜黑色素瘤的PCNA免疫染色结果时应谨慎。
