Bannister N J, Larkman A U
University Laboratory of Physiology, Oxford University, England.
J Comp Neurol. 1995 Sep 11;360(1):150-60. doi: 10.1002/cne.903600111.
The aim of this study was to provide quantitative descriptions of the dendritic branching patterns of pyramidal neurones in the CA1 region of the rat hippocampus. Thirteen adult cells were filled with biocytin and reconstructed by using the light microscope. The number of basal trees arising from the soma of each cell ranged from two to eight. There was wide variation in the number of terminal segments per tree. Six cells had single apical trunks, and seven had trunks that bifurcated in stratum radiatum. The number of apical oblique trees ranged from nine to 30, with each tree usually showing a lower degree of branching than basal trees. Basal and oblique trees had similar branching patterns, with the majority of branch points occurring close to the origin of the tree. Both basal and oblique terminal segments were generally much longer than intermediate segments and constituted up to 90% of the combined dendritic length of the tree. The branching pattern of the apical tuft was different, with many relatively long intermediate segments; terminal segments contributed only some 66% of the combined dendritic length of these trees. The mean total combined dendritic length for six adult cells reconstructed and measured completely was 11,900 +/- 1,000 microns (standard deviation). The relative proportions of the different parts of the dendritic system, although not the total dendritic length, were correlated with the location of the soma relative to the cell body layer. Cells with somata close to the stratum pyramidale/stratum radiatum border had more dendrites terminating in stratum radiatum and fewer in stratum oriens than cells with somata further from it.
本研究的目的是对大鼠海马体CA1区锥体细胞的树突分支模式进行定量描述。13个成年细胞用生物素填充，并通过光学显微镜进行重建。每个细胞胞体发出的基树突数量从2到8个不等。每棵树的终末节段数量差异很大。6个细胞有单个顶树突干，7个细胞的树突干在辐射层分叉。顶斜树突的数量从9到30个不等，每棵树通常比基树突的分支程度低。基树突和斜树突具有相似的分支模式，大多数分支点靠近树突的起始处。基树突和斜树突的终末节段通常比中间节段长得多，占树突总长度的90%。顶树突簇的分支模式不同，有许多相对较长的中间节段；终末节段仅占这些树突总长度的约66%。对6个完全重建和测量成年细胞的树突总长度平均值为11,900±1,000微米（标准差）。树突系统不同部分的相对比例，虽然不是树突总长度，但与胞体相对于细胞体层的位置相关。胞体靠近锥体层/辐射层边界的细胞，其树突在辐射层终止的比在原层终止的多，而胞体离该边界较远的细胞则相反。
