Dragunow M, Preston K, Dodd J, Young D, Lawlor P, Christie D
Department of Pharmacology, University of Auckland, New Zealand.
Brain Res Mol Brain Res. 1995 Sep;32(2):279-90. doi: 10.1016/0169-328x(95)00088-a.
Clusterin is a protein that has been implicated in cell death and remodelling in a number of different tissues. To further investigate the role of clusterin in nerve cell death its expression was measured in the rat brain at various times after status epilepticus (SE) induced by 1 h of hippocampal stimulation, by using in situ hybridization, immunocytochemistry, and immunoblotting. SE lead to a dramatic time-dependent increase in clusterin mRNA in non-nerve cells resembling astrocytes in the hippocampus beginning after 24 h. There was also an earlier induction of clusterin mRNA in dentate granule cells, that survive SE. Only a low mRNA signal was observed over the CA1 pyramidal cells, which die after SE. In contrast to these mRNA results, massive clusterin-like immunoreactivity was observed in CA1 pyramidal cells and dentate hilar neurons (and both of these neuronal populations die after SE), but not in dentate granule cells. We speculate that astrocytes produce clusterin after SE and that the clusterin is then secreted and taken up by hippocampal neurons destined to die. Thus, the role of clusterin in nerve cell death/ regeneration warrants further investigation.
簇集素是一种与多种不同组织中的细胞死亡和重塑有关的蛋白质。为了进一步研究簇集素在神经细胞死亡中的作用，通过原位杂交、免疫细胞化学和免疫印迹法，在海马体刺激1小时诱导癫痫持续状态（SE）后的不同时间，测量大鼠脑中簇集素的表达。SE导致海马体中类似于星形胶质细胞的非神经细胞中簇集素mRNA在24小时后开始出现显著的时间依赖性增加。在齿状颗粒细胞中也有簇集素mRNA的早期诱导，这些细胞在SE后存活。在SE后死亡的CA1锥体细胞中仅观察到低mRNA信号。与这些mRNA结果相反，在CA1锥体细胞和齿状门区神经元中观察到大量的簇集素样免疫反应性（这两个神经元群体在SE后均死亡），但在齿状颗粒细胞中未观察到。我们推测星形胶质细胞在SE后产生簇集素，然后簇集素被分泌并被注定死亡的海马神经元摄取。因此，簇集素在神经细胞死亡/再生中的作用值得进一步研究。
