利用1H NMR测定骨骼肌中乳酸的13C富集情况：使用双量子滤波器同时编辑13C耦合和13C未耦合的甲基质子共振信号。
1H NMR determination of lactate 13C-enrichment in skeletal muscle: using a double quantum filter for the simultaneous editing of 13C-coupled and 13C-uncoupled methyl protons resonance.
作者信息
Bloch G, Jouvensal L, Carlier P G
机构信息
CEA, Service Hospitalier Fréderic Joliot, Département de Recherche en Imagerie, Pharmacologie et Physiologie, Orsay, France.
出版信息
Magn Reson Med. 1995 Sep;34(3):353-8. doi: 10.1002/mrm.1910340312.
1H NMR simultaneous editing of 13C-coupled and 13C-uncoupled methyl protons resonance, using the selection of double quantum coherences by a gradient pulse, was analyzed in vitro and demonstrated in situ on the hindlimb of an exercised rat model postmortem. In vitro calibration showed agreement with theoretical analysis. High-resolution NMR of muscle extract confirmed the accuracy of the lactate 13C-enrichment calculated using the in situ NMR data and the calibration factor obtained in vitro.
利用梯度脉冲选择双量子相干性对与¹³C耦合和未耦合的甲基质子共振进行¹H NMR同步编辑，在体外进行了分析，并在死后的运动大鼠模型后肢上进行了原位验证。体外校准结果与理论分析一致。肌肉提取物的高分辨率NMR证实了使用原位NMR数据和体外获得的校准因子计算出的乳酸¹³C富集的准确性。
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