Cheng T J, Christiani D C, Liber H L, Wain J C, Xu X, Wiencke J K, Kelsey K T
Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115, USA.
Mutat Res. 1995 Nov;332(1-2):109-18. doi: 10.1016/0027-5107(95)00161-8.
A clonal assay to determine the mutant frequency (MF) at the hypoxanthine-guanine phosphoribosyl transferase (hprt) locus in human lymphocytes has been used by a number of investigators to study exposure to mutagens and carcinogens in a variety of populations. We have studied hprt MF in 106 subjects (40 controls and 66 cases) enrolled in a case-control investigation of lung cancer. Epidemiological data collected included smoking history, intake of dietary micronutrients, and occupational and environmental exposures as well as medical history, all obtained from an interviewer-administered questionnaire. All subjects were also genotyped for the known polymorphism in glutathione S-transferase class mu (GST-mu). In analysis of cases and controls, hprt MF was not associated with age, smoking, the polymorphism in GST mu, dietary intake, occupational exposures, family history of cancer or usage of medications. Since MF and cloning efficiency (CE) are not independent when CE is low, further analysis in cases and controls with a CE greater than or equal to 30% (27 cases and 22 controls) was also conducted. In analysis of controls, hprt MF increased with age and was inversely associated with intake of folate and vitamins A and C. The presence of lung cancer was not associated with hprt MF. Thus, our study supports the previous observation that dietary components may affect the MF at the hprt locus.
许多研究人员采用一种克隆分析方法来测定人类淋巴细胞中次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶（hprt）位点的突变频率（MF），以研究不同人群接触诱变剂和致癌物的情况。我们对参与肺癌病例对照研究的106名受试者（40名对照和66名病例）的hprt MF进行了研究。收集的流行病学数据包括吸烟史、膳食微量营养素摄入、职业和环境暴露以及病史，所有这些都是通过访员管理的问卷获得的。所有受试者还针对谷胱甘肽S - 转移酶μ类（GST - μ）中已知的多态性进行了基因分型。在病例和对照的分析中，hprt MF与年龄、吸烟、GST μ的多态性、膳食摄入、职业暴露、癌症家族史或药物使用无关。由于当克隆效率（CE）较低时，MF和CE并非相互独立，因此我们还对CE大于或等于30%的病例和对照（27例病例和22例对照）进行了进一步分析。在对照分析中，hprt MF随年龄增加而升高，并且与叶酸以及维生素A和C的摄入量呈负相关。肺癌的存在与hprt MF无关。因此，我们的研究支持了先前的观察结果，即膳食成分可能会影响hprt位点的MF。
