Nagashima G, Aoyagi M, Wakimoto H, Tamaki M, Ohno K, Hirakawa K
Department of Neurosurgery, Tokyo Medical and Dental University, Japan.
Neurosurgery. 1995 Sep;37(3):478-82; discussion 483. doi: 10.1227/00006123-199509000-00016.
Female sex steroids may play a role in the proliferation of meningiomas. We investigated the progesterone receptor (PgR) immunoreactivities and the Ki-67 labeling indices in the formalin-fixed, paraffin-embedded sections of meningiomas from 39 patients. After autoclave pretreatment of the sections (which were immersed in a citrate buffer), the sections were incubated with the monoclonal antibody for the PgR and the MIB-1 monoclonal antibody for the Ki-67 antigen. In the meningiomas studied, the immunoreactivity for the PgR was moderately to strongly positive in 51%, weakly positive in 21%, and negative in 28%. The nuclear staining for the PgR was clear, and no tumors were positive for the estrogen receptor. The Ki-67 labeling indices of the PgR-positive meningiomas (mean +/- standard deviation, 2.35 +/- 2.12%) were significantly lower than those of the PgR-negative meningiomas (6.53 +/- 4.83%) (P < 0.05). Two meningiomas that had recurred more than once showed high Ki-67 labeling indices and negative immunostaining for the PgR. These findings indicate that the PgR status may be closely related to the growth potentials of the meningiomas. Our results confirm that the immunodetection of the PgR and the Ki-67 antigen on the paraffin sections of meningiomas provides a practical tool for estimating the biological behavior of the meningiomas.
女性甾体激素可能在脑膜瘤的增殖中起作用。我们研究了39例患者脑膜瘤的福尔马林固定、石蜡包埋切片中的孕激素受体（PgR）免疫反应性和Ki-67标记指数。将切片（浸入柠檬酸盐缓冲液中）进行高压灭菌预处理后，用针对PgR的单克隆抗体和针对Ki-67抗原的MIB-1单克隆抗体孵育切片。在所研究的脑膜瘤中，PgR免疫反应性中度至强阳性占51%，弱阳性占21%，阴性占28%。PgR的核染色清晰，且无肿瘤雌激素受体呈阳性。PgR阳性脑膜瘤的Ki-67标记指数（平均值±标准差，2.35±2.12%）显著低于PgR阴性脑膜瘤（6.53±4.83%）（P<0.05）。有两次以上复发的两个脑膜瘤显示Ki-67标记指数高且PgR免疫染色阴性。这些发现表明PgR状态可能与脑膜瘤的生长潜能密切相关。我们的结果证实，在脑膜瘤石蜡切片上对PgR和Ki-67抗原进行免疫检测为评估脑膜瘤的生物学行为提供了一种实用工具。
