由冯雷克林霍增氏神经纤维瘤病（NF1）基因产生的神经纤维瘤蛋白亚型的两种mRNA：在人类星形细胞瘤中的分析

The two types of mRNAs for neurofibromin isoforms produced by von Recklinghausen neurofibromatosis (NF1) gene: analysis in human astrocytic tumors.

作者信息

Tokuyama T, Uemura K, Fujita M

机构信息

Department of Biochemistry, Hamamatsu University School of Medicine, Japan.

出版信息

Neurosci Lett. 1995 Aug 25;196(3):189-92. doi: 10.1016/0304-3940(95)11875-w.

DOI:10.1016/0304-3940(95)11875-w

PMID:7501280

Abstract

Two types of mRNAs for neurofibromin isoforms, which are produced by Von Recklinghausen neurofibromatosis (NF1) gene, have so far been identified. In the present study, we analyzed the differential expression of the two NF1 mRNAs in 16 cases of human astrocytic tumors and in surrounding normal tissues by the RNA-polymerase chain reaction. Astrocytic tumors predominantly expressed type II NF1 mRNA, whereas it was type I isoform that was predominantly expressed in the normal tissues. These results suggested that the increased type II NF1 protein might play an important role in the growth of astrocytic tumors.

摘要

迄今为止，已鉴定出由冯·雷克林霍增氏神经纤维瘤病（NF1）基因产生的两种神经纤维瘤蛋白亚型的mRNA。在本研究中，我们通过RNA聚合酶链反应分析了16例人类星形细胞瘤及周围正常组织中两种NF1 mRNA的差异表达。星形细胞瘤主要表达II型NF1 mRNA，而正常组织中主要表达的是I型亚型。这些结果表明，II型NF1蛋白的增加可能在星形细胞瘤的生长中起重要作用。