Lin S H, Miyata S, Itoh T, Kawarabayashi T, Nakashima T, Kiyohara T
Department of Applied Biology, Kyoto Institute of Technology, Japan.
Neurosci Res. 1995 Aug;23(1):29-34. doi: 10.1016/0168-0102(95)90007-1.
Changes in Fos expression of magnocellular neurons in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) of the rat hypothalamus were investigated using immunohistochemistry during pregnancy, parturition and lactation. Quantitative morphometrical analysis revealed that Fos-positive cells in the hypothalamic magnocellular neurons were rarely seen in days 10 and 20 pregnant rats, however, significantly numerous Fos-positive cells were found in parturient rats. The number of Fos-positive cells was drastically decreased within a few days after parturition. Moreover, it was found using dual immunohistochemistry that the percentage of Fos-positive cells in vasopressin (AVP) magnocellular neurons of the SON was higher than that of the PVN in parturient rats, although oxytocin (OXT) magnocellular neurons showed the same percentage of Fos-positive cells between the SON and PVN. These results demonstrate that the hypothalamic magnocellular neurons express Fos during a limited period after parturition, and Fos expression in AVP magnocellular neurons is heterogeneous between the SON and PVN.
采用免疫组织化学方法，研究了大鼠下丘脑视上核（SON）和室旁核（PVN）大细胞神经元在妊娠、分娩和哺乳期Fos表达的变化。定量形态学分析显示，妊娠第10天和第20天的大鼠下丘脑大细胞神经元中很少见到Fos阳性细胞，然而，在分娩大鼠中发现了大量的Fos阳性细胞。产后几天内，Fos阳性细胞数量急剧减少。此外，采用双重免疫组织化学方法发现，分娩大鼠中，SON中血管加压素（AVP）大细胞神经元的Fos阳性细胞百分比高于PVN，尽管催产素（OXT）大细胞神经元在SON和PVN中的Fos阳性细胞百分比相同。这些结果表明，下丘脑大细胞神经元在分娩后的有限时间内表达Fos，并且AVP大细胞神经元中Fos的表达在SON和PVN之间是异质性的。
