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果蝇反转录转座子在体内外由RNA聚合酶II和III进行转录的复杂模式。

Complex patterns of transcription of a Drosophila retrotransposon in vivo and in vitro by RNA polymerases II and III.

作者信息

Arkhipova I R

机构信息

Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138-2092, USA.

出版信息

Nucleic Acids Res. 1995 Nov 11;23(21):4480-7. doi: 10.1093/nar/23.21.4480.

DOI:10.1093/nar/23.21.4480
PMID:7501473
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307407/
Abstract

The mdg1 retrovirus-like retrotransposon of Drosophila melanogaster was found to possess a complex promoter which can be transcribed by both RNA polymerases II and III (pol II and pol III). Pol III transcription, which is not typical of protein-coding genes, is driven by the sequences located in the long terminal repeat (LTR) of mdg1, predominantly within the transcribed region and is initiated 10 bp upstream from the regular pol II RNA start site. The pol III RNA start site is observed not only in in vitro transcription reactions, but also in total RNA isolated from tissue culture cells, larvae, pupae and adult flies. A possible role of pol III transcription in mechanisms controlling the expression of full-length mdg1-encoded transcripts in the developing fly, which are apparently relaxed in cell culture, is discussed.

摘要

人们发现，果蝇的mdg1逆转录病毒样反转录转座子拥有一个复杂的启动子，该启动子可由RNA聚合酶II和III（pol II和pol III）转录。pol III转录并非蛋白质编码基因的典型特征，它由mdg1长末端重复序列（LTR）中的序列驱动，主要位于转录区域内，并在常规pol II RNA起始位点上游10 bp处起始。pol III RNA起始位点不仅在体外转录反应中可以观察到，在从组织培养细胞、幼虫、蛹和成年果蝇中分离的总RNA中也能观察到。本文讨论了pol III转录在发育中的果蝇中控制全长mdg1编码转录本表达机制中的可能作用，这种机制在细胞培养中显然较为宽松。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/b3717b175bab/nar00021-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/685678d4e089/nar00021-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/33381c78b8f3/nar00021-0264-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/48774fb32900/nar00021-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/b7165fe6afc5/nar00021-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/fc5b943d09db/nar00021-0266-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/c7f1591bbf31/nar00021-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/e0ad6dc61bbf/nar00021-0267-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/b3717b175bab/nar00021-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/685678d4e089/nar00021-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/33381c78b8f3/nar00021-0264-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/48774fb32900/nar00021-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/b7165fe6afc5/nar00021-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/fc5b943d09db/nar00021-0266-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/c7f1591bbf31/nar00021-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/e0ad6dc61bbf/nar00021-0267-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db54/307407/b3717b175bab/nar00021-0268-a.jpg

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Insect Biochem Mol Biol. 1993 Jan;23(1):81-90. doi: 10.1016/0965-1748(93)90085-7.
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The 5' untranslated region of the I factor, a long interspersed nuclear element-like retrotransposon of Drosophila melanogaster, contains an internal promoter and sequences that regulate expression.I因子的5'非翻译区是黑腹果蝇中一种长散在核元件样逆转座子，它包含一个内部启动子和调控表达的序列。
Mol Cell Biol. 1993 Feb;13(2):1042-50. doi: 10.1128/mcb.13.2.1042-1050.1993.
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TBP, a universal eukaryotic transcription factor?
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Functional dissection of two promoters that control sense and antisense transcription of Drosophila melanogaster F elements.控制黑腹果蝇F元件正义和反义转录的两个启动子的功能剖析。
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