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Rapid purification of recombinant Taq DNA polymerase by freezing and high temperature thawing of bacterial expression cultures.

作者信息

Grimm E, Arbuthnot P

机构信息

Medical Biochemistry Department, University of the Witwatersrand Medical School, Parktown, South Africa.

出版信息

Nucleic Acids Res. 1995 Nov 11;23(21):4518-9. doi: 10.1093/nar/23.21.4518.

DOI:10.1093/nar/23.21.4518
PMID:7501478
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307412/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f27f/307412/7c040a1df23f/nar00021-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f27f/307412/7c040a1df23f/nar00021-0301-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f27f/307412/7c040a1df23f/nar00021-0301-a.jpg

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本文引用的文献

1
Rapid purification of high-activity Taq DNA polymerase.高活性Taq DNA聚合酶的快速纯化
Nucleic Acids Res. 1993 Oct 11;21(20):4850-1. doi: 10.1093/nar/21.20.4850.
2
Use of T7 RNA polymerase to direct expression of cloned genes.使用T7 RNA聚合酶指导克隆基因的表达。
Methods Enzymol. 1990;185:60-89. doi: 10.1016/0076-6879(90)85008-c.
3
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli.在大肠杆菌中表达的嗜热水生栖热菌DNA聚合酶的纯化。
大肠杆菌蛋白质组中的底物模糊酶为同一问题提供了不同的进化解决方案。
Mol Biol Evol. 2013 Sep;30(9):2001-12. doi: 10.1093/molbev/mst105. Epub 2013 May 31.
4
Optimization of Taq DNA polymerase enzyme expression in Escherichia coli.大肠杆菌中Taq DNA聚合酶表达的优化
Adv Biomed Res. 2012;1:82. doi: 10.4103/2277-9175.103004. Epub 2012 Oct 31.
5
Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles.在低变性温度下进行的连接介导PCR——PCR熔解曲线
Nucleic Acids Res. 2003 Sep 15;31(18):e114. doi: 10.1093/nar/gng116.
6
DNA polymerase active site is highly mutable: evolutionary consequences.DNA聚合酶活性位点具有高度可变性:进化后果。
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5095-100. doi: 10.1073/pnas.97.10.5095.
7
In vitro evolution of thermostable p53 variants.热稳定p53变体的体外进化
Protein Sci. 1999 Apr;8(4):731-40. doi: 10.1110/ps.8.4.731.
Anal Biochem. 1990 Dec;191(2):396-400. doi: 10.1016/0003-2697(90)90238-5.