Kubota Y, Fujinami K, Uemura H, Dobashi Y, Miyamoto H, Iwasaki Y, Kitamura H, Shuin T
Department of Urology, Yokohama City University, School of Medicine, Japan.
Prostate. 1995 Dec;27(6):314-20. doi: 10.1002/pros.2990270604.
Structural alterations in the entire coding regions (exons 1 to 27) of the retinoblastoma (RB) gene in primary human prostate cancers were investigated, using polymerase chain reaction and single strand conformational polymorphism analysis of RNA. Of 25 samples obtained from patients, four (16.4%) were found to have RB alterations. DNA sequencing of the PCR products revealed point mutations resulting in single amino-acid substitutions of exons 6 and 19 in two cases, and base deletions of exons 8 and 17 in two cases. Two of four cases with RB mutations were moderately differentiated localized tumors and other two with RB mutations were poorly differentiated tumors with metastases. Our results suggest that RB gene mutation is involved in progression steps of prostate carcinogenesis.
利用聚合酶链反应和RNA的单链构象多态性分析，对原发性人类前列腺癌中视网膜母细胞瘤（RB）基因整个编码区（外显子1至27）的结构改变进行了研究。在从患者获取的25个样本中，发现4个（16.4%）存在RB改变。对PCR产物进行DNA测序显示，两例出现导致外显子6和19单氨基酸替换的点突变，两例出现外显子8和17的碱基缺失。4例发生RB突变的病例中，两例为中度分化的局限性肿瘤，另两例为伴有转移的低分化肿瘤。我们的结果表明，RB基因突变参与了前列腺癌发生的进展过程。
