通过微量薄层色谱法和荧光法快速检测尿液中的苯丙胺。
Rapid detection of amphetamine in urine by micro thin-layer chromatography and fluorescence.
作者信息
Decker W J, Thompson J D
出版信息
Clin Toxicol. 1978 Dec;13(5):545-9. doi: 10.3109/15563657808988260.
A rapid, inexpensive, and simple screening procedure for the detection of amphetamine abuse was developed for use by laboratories without sophisticated equipment. A small volume of extract from a pH-adjusted urine specimen is used to spot a high-resolution micro TLC plate. The developed TLC plate is sprayed with a solution of fluorescamine in dry acetone. When viewed under ultraviolet illumination, amphetamines and other compounds with a primary amino group complexed with fluorescamine appear as greenish or bluish-white fluorescent spots. Secondary or tertiary amines do not react with fluorescamine. About 20 min is required to perform the procedure; the lower limit of detectability is approximately 100 ng/ml urine.
开发了一种快速、廉价且简单的检测苯丙胺滥用的筛查程序，供没有精密设备的实验室使用。从经pH调节的尿液标本中取少量提取物，点样于高分辨率微量薄层色谱板上。展开后的薄层色谱板用荧光胺的干燥丙酮溶液喷雾。在紫外光照射下观察时，与荧光胺络合的苯丙胺和其他具有伯氨基的化合物呈现出绿色或蓝白色荧光斑点。仲胺或叔胺不与荧光胺反应。该程序大约需要20分钟；可检测下限约为100 ng/ml尿液。
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