聚苯乙烯板中孔的预处理对某些人血清蛋白吸附的影响。

Effect of pretreatment of wells in polystyrene plates on adsorption of some human serum proteins.

作者信息

Kochanowska I E, Rapak A, Szewczuk A

机构信息

Biochemical Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.

出版信息

Arch Immunol Ther Exp (Warsz). 1994;42(2):135-9.

PMID:7503647

Abstract

Coating of Immulon polystyrene plates with 0.2% casein in phosphate buffered saline (PBS) completely abolishes adsorption of human serum proteins to these plates. However, pretreatment of such plates with PBS or 0.15 M NaCl in deionized water (10 microS) before coating makes possible adsorption of human immunoglobulins G (HIgG) and M but not serum albumin (HSA) and transferrin (HTrf). Effect of pretreatment with PBS on adsorption of HIgG is long-term and rather stable. Results of experiments with radioiodinated HIgG, mouse IgG, HSA and human chorionic gonadotropin confirm the peculiar effect of pretreatment with PBS on casein coating. Pretreatment with deionized water, instead of PBS, markedly diminish adsorption but tap or commercial spring waters (> 1000 microS), even without 0.15 M NaCl, affect adsorption similarly to PBS in deionized water. Super pure water (0.05 microS) even with NaCl used for pretreatment does not influence adsorption of HIgG to plates coated with casein. Distinct differences in adsorption of HIgG and HTrf was demonstrated using solutions for ELISA prepared from super pure, deionized and tap waters.

摘要

用磷酸盐缓冲盐水（PBS）中0.2%的酪蛋白包被Immulon聚苯乙烯板可完全消除人血清蛋白对这些板的吸附。然而，在包被前用PBS或去离子水中的0.15 M NaCl（10微西门子）对这种板进行预处理，可使人免疫球蛋白G（HIgG）和M吸附，但血清白蛋白（HSA）和转铁蛋白（HTrf）不吸附。用PBS预处理对HIgG吸附的影响是长期且相当稳定的。用放射性碘化HIgG、小鼠IgG、HSA和人绒毛膜促性腺激素进行的实验结果证实了用PBS预处理对酪蛋白包被的特殊作用。用去离子水而非PBS预处理会显著减少吸附，但自来水或市售泉水（>1000微西门子），即使没有0.15 M NaCl，对吸附的影响与去离子水中的PBS类似。即使在预处理中使用了NaCl，超纯水（0.05微西门子）也不会影响HIgG对用酪蛋白包被的板的吸附。使用由超纯水、去离子水和自来水制备的ELISA溶液，证明了HIgG和HTrf吸附存在明显差异。