Lensen H G, Breemhaar W, Smolders C A, Feijen J
J Chromatogr. 1986 Apr 11;376:191-8.
The competitive adsorption of human serum albumin (HSA), human immuno-gamma-globulin (HIgG) and human fibrinogen (HFb) onto polystyrene (PS) at 20 degrees C and a pH of 7.35 (phosphate-buffered saline) was studied. Protein adsorption was studied using enzyme immunoassay. The results obtained with the immunoassay were compared with those obtained using radiolabelled proteins. Recent studies revealed that the adsorption behaviour of radiolabelled proteins onto surfaces differs from that of the non-labelled proteins, which may lead to misinterpretation of adsorption data. Differences in the adsorption behaviour of the labelled proteins as compared to non-labelled proteins can possibly be explained by the formation of modified proteins during the labelling procedure as shown by ion-exchange high-performance liquid chromatography (HPLC). The competitive adsorption of HSA, HIgG and HFb onto a PS latex was studied by measuring the depletion of proteins in solution. The decrease in protein concentration in solution was determined by HPLC techniques. A strong preferential adsorption of HFb was observed with maximum adsorption values of 0.6 micrograms/cm2.
研究了人血清白蛋白（HSA）、人免疫γ球蛋白（HIgG）和人纤维蛋白原（HFb）在20℃、pH值为7.35（磷酸盐缓冲盐水）条件下在聚苯乙烯（PS）上的竞争性吸附。使用酶免疫测定法研究蛋白质吸附。将免疫测定法得到的结果与使用放射性标记蛋白质得到的结果进行比较。最近的研究表明，放射性标记蛋白质在表面的吸附行为与未标记蛋白质不同，这可能导致对吸附数据的错误解读。与未标记蛋白质相比，标记蛋白质吸附行为的差异可能是由于在标记过程中形成了修饰蛋白质，如离子交换高效液相色谱（HPLC）所示。通过测量溶液中蛋白质的消耗来研究HSA、HIgG和HFb在PS乳胶上的竞争性吸附。溶液中蛋白质浓度的降低通过HPLC技术测定。观察到HFb有强烈的优先吸附，最大吸附值为0.6微克/平方厘米。
