Rieneck K, Diamant M, Haahr P M, Schönharting M, Bendtzen K
Laboratory of Medical Immunology 7544, Rigshospitalet, Copenhagen, Denmark.
Immunol Lett. 1993 Aug;37(2-3):131-8. doi: 10.1016/0165-2478(93)90022-t.
Pentoxifylline (PTX), a methylxanthine derivative and phosphodiesterase inhibitor, is known to influence production and/or function of some cytokines. We examined the effect of PTX on the in vitro expression of cytokine genes using endotoxin- or phytohaemagglutinin (PHA)-stimulated human blood mononuclear cells. The expression of tumour necrosis factor (TNF)alpha, TNF beta interleukin (IL)-2 and interferon (IFN)gamma was inhibited by PTX in a dose-dependent manner, whereas expression of IL-1 alpha, IL-1 beta, and IL-6 was unaffected at concentrations up to 300 microM of PTX. The amount of TNF beta mRNA in PHA-stimulated blood mononuclear cells was reduced by PTX. Finally, PTX stimulated PHA-induced cell proliferation whereas antigen-induced cell proliferation was inhibited in the presence of PTX. The PTX analogues HWA-138 and A-802715 inhibited TNF alpha mRNA expression from endotoxin-stimulated mononuclear cells. These data suggest that PTX-analogues affect the in vitro immune response at different target points and that the response depends upon the respective triggering mechanism(s).
己酮可可碱（PTX）是一种甲基黄嘌呤衍生物和磷酸二酯酶抑制剂，已知其会影响某些细胞因子的产生和/或功能。我们使用内毒素或植物血凝素（PHA）刺激的人血单核细胞研究了PTX对细胞因子基因体外表达的影响。PTX以剂量依赖的方式抑制肿瘤坏死因子（TNF）α、TNFβ、白细胞介素（IL）-2和干扰素（IFN）γ的表达，而在PTX浓度高达300微摩尔时，IL-1α、IL-1β和IL-6的表达不受影响。PTX降低了PHA刺激的血单核细胞中TNFβmRNA的量。最后，PTX刺激PHA诱导的细胞增殖，而在PTX存在的情况下，抗原诱导的细胞增殖受到抑制。PTX类似物HWA-138和A-802715抑制内毒素刺激的单核细胞中TNFαmRNA的表达。这些数据表明，PTX类似物在不同靶点影响体外免疫反应，且反应取决于各自的触发机制。
