核磁共振证据表明RNA茎环结构参与大肠杆菌色氨酸操纵子的转录衰减。
NMR evidence for the RNA stem-loop structure involved in the transcription attenuation of E. coli trp operon.
作者信息
Ramesh V
机构信息
Department of Biochemistry, University of Leicester, UK.
出版信息
Nucleic Acids Res. 1993 Nov 25;21(23):5485-8. doi: 10.1093/nar/21.23.5485.
Abstract
High field 1H-NMR studies of a synthetic 21-mer RNA fragment, corresponding to residues +114 to +134 within the trp leader mRNA transcript, have been carried out. Seven well resolved imino proton resonances corresponding to six C-G and one A-U hydrogen bonded base pairs, together with their characteristic NOE patterns can be identified in the NMR spectrum. This experimental result provides direct evidence for the postulated stem-loop secondary structure, 3:4, which has been reported to act as a transcription termination signal for RNA polymerase.
摘要
已经对一个合成的21聚体RNA片段进行了高场1H-NMR研究，该片段对应于色氨酸前导mRNA转录本中的+114至+134位残基。在NMR光谱中可以识别出七个分辨率良好的亚氨基质子共振峰，它们对应于六个C-G和一个A-U氢键碱基对，以及它们的特征性NOE模式。这一实验结果为假定的3:4茎环二级结构提供了直接证据，据报道该结构可作为RNA聚合酶的转录终止信号。
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