Jia R, Hanafusa H
Rockefeller University, New York, New York 10021-6399.
J Biol Chem. 1994 Jan 21;269(3):1839-44.
v-ryk is the oncogene in the avian acute oncogenic retrovirus RPL30, its cellular counterpart, c-ryk, is described in this report. To avoid the confusion caused by another gene with the same name, we renamed v-ryk to be v-eyk, thereby changing the proto-oncogene's name to c-eyk. c-eyk is expressed highly in chicken spleen and moderately in many other tissues including embryonic tissues. Its cDNA is 3061 base pairs encoding a receptor-type protein tyrosine kinase (PTK) of 974 amino acids. Compared to c-Eyk, v-Eyk is a truncated PTK lacking the extracellular and transmembrane regions of c-Eyk in addition to two amino acid changes. c-Eyk has two C2-type Ig domains and two FN-III domains in its extracellular region, forming a new subfamily of receptor-type PTK together with UFO/Axl/Ark. As suggested by Ig/Fn-III domains in many cell adhesion molecules, this subfamily of PTK may mediate cell-cell interaction.
v-ryk是禽急性致癌逆转录病毒RPL30中的癌基因，其细胞对应物c-ryk在本报告中予以描述。为避免与另一个同名基因造成混淆，我们将v-ryk重命名为v-eyk，从而将原癌基因的名称改为c-eyk。c-eyk在鸡脾脏中高表达，在包括胚胎组织在内的许多其他组织中中度表达。其cDNA为3061个碱基对，编码一个由974个氨基酸组成的受体型蛋白酪氨酸激酶（PTK）。与c-Eyk相比，v-Eyk是一个截短的PTK，除了两个氨基酸变化外，还缺少c-Eyk的细胞外和跨膜区域。c-Eyk在其细胞外区域有两个C2型免疫球蛋白结构域和两个纤连蛋白III型结构域，与UFO/Axl/Ark一起形成受体型PTK的一个新亚家族。正如许多细胞粘附分子中的免疫球蛋白/纤连蛋白III型结构域所表明的那样, 这个PTK亚家族可能介导细胞间相互作用。
